Characteristics of the adhesion of PCC® Lactobacillus fermentum VRI 003 to Peyer's patches

Seok Seong Kang, Patricia L. Conway

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The characteristics of the adhesion of PCC® Lactobacillus fermentum VRI 003 to Peyer's patches was studied in vitro. The adhesion of L. fermentum 003 was strongly inhibited in the presence of D-mannose and methyl-α-D- mannoside although other carbohydrates tested, such as N-acetyl-glucosamine, D-galactose, D-glucose and L-fucose, did not affect the adhesion. Lactobacillus fermentum 003 was shown to strongly attach to mannose immobilized on a surface using BSA, suggesting that L. fermentum 003 specifically adhered to mannose-containing molecule(s). Pretreatment of L. fermentum 003 with proteinase K and trypsin decreased the adhesive capacity and bacterial surface extracts diminished adhesion of L. fermentum 003 indicating that cell surface proteins are involved in adhesion to Peyer's patches. It was concluded that a mannose-specific protein mediated adhesion of L. fermentum 003 to the Peyer's patches.

Original languageEnglish
Pages (from-to)19-24
Number of pages6
JournalFEMS Microbiology Letters
Volume261
Issue number1
DOIs
StatePublished - Aug 2006

Keywords

  • Lactobacillus
  • Mannose-specific adhesion
  • Peyer's patches

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