TY - JOUR
T1 - Characterization of a novel repetitive secretory protein specifically expressed in the modified salivary gland of Hydropsyche sp. (Trichoptera; Hydropsychidae)
AU - Eum, Jai Hoon
AU - Yoe, Sung Moon
AU - Seo, Young R.
AU - Kang, Seok Woo
AU - Han, Sung Sik
PY - 2005/5
Y1 - 2005/5
N2 - Here, we report the cloning and characterization of a common salivary gland-specific gene, Nf-1, from late instar Hydropsyche sp. larvae, and show that the corresponding gene product is translated in the gland and secreted to the gland lumen. The deduced Nf-1 protein is primarily composed of five repetitive sequence units of 63-65 amino acids, and contains a putative signal sequence composed of 19 amino acids. Secreted Nf-1 (∼37 kDa) was localized to the gland lumen by Western blotting of gland and lumen fractions. Together, the structure, expression pattern and protein localization of Nf-1 indicate that this protein is likely to be a major component of the silk shields and nets produced by the aquatic insect, Trichoptera.
AB - Here, we report the cloning and characterization of a common salivary gland-specific gene, Nf-1, from late instar Hydropsyche sp. larvae, and show that the corresponding gene product is translated in the gland and secreted to the gland lumen. The deduced Nf-1 protein is primarily composed of five repetitive sequence units of 63-65 amino acids, and contains a putative signal sequence composed of 19 amino acids. Secreted Nf-1 (∼37 kDa) was localized to the gland lumen by Western blotting of gland and lumen fractions. Together, the structure, expression pattern and protein localization of Nf-1 indicate that this protein is likely to be a major component of the silk shields and nets produced by the aquatic insect, Trichoptera.
KW - Expressed sequence tags
KW - Modified salivary gland
KW - Nf-1 protein (Nest forming protein-1)
KW - Repetitive amino acid
KW - Trichoptera Hydropsyche sp.
UR - http://www.scopus.com/inward/record.url?scp=15944399104&partnerID=8YFLogxK
U2 - 10.1016/j.ibmb.2005.01.009
DO - 10.1016/j.ibmb.2005.01.009
M3 - Article
C2 - 15804577
AN - SCOPUS:15944399104
SN - 0965-1748
VL - 35
SP - 435
EP - 441
JO - Insect Biochemistry and Molecular Biology
JF - Insect Biochemistry and Molecular Biology
IS - 5
ER -