Characterization of monoclonal antibodies against GRIM-19, a novel IFNβ and retinoic acid-activated regulator of cell death

Jiadi Hu, Jon E. Angell, Jun Zhang, Xinrong Ma, Taegun Seo, Abhijit Raha, Jun Hayashi, Joonho Choe, Dhananjaya V. Kalvakolanu

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

A combination of interferonβ (IFNβ) and all-trans retinoic acid (IFN/RA) induces tumor cell apoptosis via some unknown mechanisms. Apoptosis is a gene-directed process that limits the proliferation of undesired cells. Several genes are required to regulate cell death in the higher-order animals. Earlier, we employed a gene expression knockout technique to isolate cell death-related genes. A novel gene, the gene associated with retinoid-interferon-induced mortality-19 (GRIM-19), was found to be essential for tumor cell death induced by IFN/RA. Here, we describe the development and characterization of three monoclonal antibodies (mAbs) against GRIM-19. GRIM-19 is present in the nucleus and cytoplasm. Its expression is induced by the IFN/RA combination. We also show that GRIM-19 inhibits the cell-transforming property of viral oncogenic protein viral IFN regulatory factor-1 (vIRF-1) via a physical interaction. mAbs developed in this study should be useful for studying the other physiologic roles of GRIM-19 and serve as a potent tool for studying tumor responses to IFN/RA therapy.

Original languageEnglish
Pages (from-to)1017-1026
Number of pages10
JournalJournal of Interferon and Cytokine Research
Volume22
Issue number10
DOIs
StatePublished - 2002

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