TY - JOUR
T1 - Chitosan-functionalized supermagnetic halloysite nanotubes for covalent laccase immobilization
AU - Kadam, Avinash A.
AU - Jang, Jiseon
AU - Jee, Seung Cheol
AU - Sung, Jung Suk
AU - Lee, Dae Sung
N1 - Publisher Copyright:
© 2018 Elsevier Ltd
PY - 2018/8/15
Y1 - 2018/8/15
N2 - Halloysite nanotubes (HNTs) were modified with supermagnetic Fe3O4 (M-HNTs) and functionalized with chitosan (CTA) (termed as M-HNTs–CTA). Furthermore, M-HNTs–CTA were cross-linked using glutaraldehyde and applied for covalent laccase immobilization (M-HNTs–CTA–Lac). Facile-synthesized modified HNTs were structurally characterized by scanning electron microscopy, high resolution transmission electron microscopy, X-ray photoelectron spectroscopy, and thermogravimetric analyses. M-HNTs–CTA–Lac exhibited 92.74 mg/g of laccase immobilization capacity and 92% of activity recovery. Biochemical properties of M-HNTs–CTA–Lac exhibited higher pH and temperature stabilities, with exceptional reusability capabilities until the 11th cycle. Moreover, M-HNTs–CTA–Lac exhibited 87% of 2,2′-azinobis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS)-mediated Direct Red 80 (DR80) decolorization. By the 11th cycle, M-HNTs–CTA–Lac exhibited 33% DR80 decolorization. Therefore, M-HNTs–CTA can function as CTA-modified supermagnetic nonreactors for immobilization of biomacromolecules. The investigated M-HNTs–CTA–Lac are thus biocompatible and environment-friendly biocatalysts for degradation of textile waste, such as DR80, and can be rapidly retrieved from aqueous solution by a magnet after decontamination of environmental pollutants.
AB - Halloysite nanotubes (HNTs) were modified with supermagnetic Fe3O4 (M-HNTs) and functionalized with chitosan (CTA) (termed as M-HNTs–CTA). Furthermore, M-HNTs–CTA were cross-linked using glutaraldehyde and applied for covalent laccase immobilization (M-HNTs–CTA–Lac). Facile-synthesized modified HNTs were structurally characterized by scanning electron microscopy, high resolution transmission electron microscopy, X-ray photoelectron spectroscopy, and thermogravimetric analyses. M-HNTs–CTA–Lac exhibited 92.74 mg/g of laccase immobilization capacity and 92% of activity recovery. Biochemical properties of M-HNTs–CTA–Lac exhibited higher pH and temperature stabilities, with exceptional reusability capabilities until the 11th cycle. Moreover, M-HNTs–CTA–Lac exhibited 87% of 2,2′-azinobis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS)-mediated Direct Red 80 (DR80) decolorization. By the 11th cycle, M-HNTs–CTA–Lac exhibited 33% DR80 decolorization. Therefore, M-HNTs–CTA can function as CTA-modified supermagnetic nonreactors for immobilization of biomacromolecules. The investigated M-HNTs–CTA–Lac are thus biocompatible and environment-friendly biocatalysts for degradation of textile waste, such as DR80, and can be rapidly retrieved from aqueous solution by a magnet after decontamination of environmental pollutants.
KW - Chitosan
KW - Halloysite nanotubes
KW - Laccase
KW - Laccase-loaded nanotubular reactors
KW - Nanobiocatalysis
KW - Sustainable environmental cleanup
UR - http://www.scopus.com/inward/record.url?scp=85045581704&partnerID=8YFLogxK
U2 - 10.1016/j.carbpol.2018.04.046
DO - 10.1016/j.carbpol.2018.04.046
M3 - Article
C2 - 29801831
AN - SCOPUS:85045581704
SN - 0144-8617
VL - 194
SP - 208
EP - 216
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
ER -