TY - JOUR
T1 - Chlortetracycline-functionalized silver nanoparticles as a colorimetric probe for aminoglycosides
T2 - Ultrasensitive determination of Kanamycin and streptomycin
AU - Saratale, Ganesh Dattatraya
AU - Saratale, Rijuta Ganesh
AU - Ghodake, Gajanan
AU - Shinde, Surendra
AU - Kim, Dae Young
AU - Alyousef, Abdullah A.
AU - Arshad, Mohammed
AU - Syed, Asad
AU - Pant, Deepak
AU - Shin, Han Seung
N1 - Publisher Copyright:
© 2020 by the authors.
PY - 2020/5
Y1 - 2020/5
N2 - Aminoglycosides (AMGs) have been extensively used to treat infectious diseases caused by Gram-negative bacteria in livestock and humans. A selective and sensitive colorimetric probe for the determination of streptomycin and kanamycin was proposed based on chlortetracycline-coated silver nanoparticles (AgNPs–CTC) as the sensing element. Almost all of the tested aminoglycoside antibiotics can rapidly induce the aggregation of AgNPs, along with a color change from yellow to orange/red. The selective detection of aminoglycoside antibiotics, including tobramycin, streptomycin, amikacin, gentamicin, neomycin, and kanamycin, with other types of antibiotics, can be achieved by ultraviolet (UV) spectroscopy. This developed colorimetric assay has ability to detect various AMGs using in-depth surface plasmon resonance (SPR) studies. With this determination of streptomycin and kanamycin was achieved at the picomolar level (pM) by using a UV–visible spectrophotometer. Under aqueous conditions, the linear range of the colorimetric sensor for streptomycin and kanamycin was 1000–1,1000 and 120–480 pM, respectively. The corresponding limit of detection was 2000 pM and 120 pM, respectively. Thus, the validated dual colorimetric and ratiometric method can find various analytical applications for the ultrasensitive and rapid detection of AMG antibiotics in water samples.
AB - Aminoglycosides (AMGs) have been extensively used to treat infectious diseases caused by Gram-negative bacteria in livestock and humans. A selective and sensitive colorimetric probe for the determination of streptomycin and kanamycin was proposed based on chlortetracycline-coated silver nanoparticles (AgNPs–CTC) as the sensing element. Almost all of the tested aminoglycoside antibiotics can rapidly induce the aggregation of AgNPs, along with a color change from yellow to orange/red. The selective detection of aminoglycoside antibiotics, including tobramycin, streptomycin, amikacin, gentamicin, neomycin, and kanamycin, with other types of antibiotics, can be achieved by ultraviolet (UV) spectroscopy. This developed colorimetric assay has ability to detect various AMGs using in-depth surface plasmon resonance (SPR) studies. With this determination of streptomycin and kanamycin was achieved at the picomolar level (pM) by using a UV–visible spectrophotometer. Under aqueous conditions, the linear range of the colorimetric sensor for streptomycin and kanamycin was 1000–1,1000 and 120–480 pM, respectively. The corresponding limit of detection was 2000 pM and 120 pM, respectively. Thus, the validated dual colorimetric and ratiometric method can find various analytical applications for the ultrasensitive and rapid detection of AMG antibiotics in water samples.
KW - Aminoglycoside antibiotics
KW - Chlorotetracycline antibiotics
KW - Picomolar level sensitivity
KW - Silver nanoparticles
KW - Water samples
UR - http://www.scopus.com/inward/record.url?scp=85085283148&partnerID=8YFLogxK
U2 - 10.3390/nano10050997
DO - 10.3390/nano10050997
M3 - Article
AN - SCOPUS:85085283148
SN - 2079-4991
VL - 10
JO - Nanomaterials
JF - Nanomaterials
IS - 5
M1 - 997
ER -