Abstract
Nucleus pulposus(NP) cells located at intervertebral disc are chondrocyte-like cells secreting cartilagespecific matrix molecules such as type 2 collagen and aggrecan. NP cells transplantation is used for recovery of damaged intervertebral disc. However, it is difficult to obtain sufficient quantity of NP cells from donor for cell therapy. Recently, adipose derived stromal cells(ASCs) as alternative cell sources have been extensively studied for cells therapy because ASCs can differentiate into several lineages including chondrogenesis. In this study, we sought to optimize the co-culture condition of NP cells and human ASCs for ASC differentiation. Media condition for NP cells and ASCs co-culture was determined by comparing proliferation and differentiation. After that, various cell ratios between NP cells and ASCs(0, 25, 50, 75, 100% ASC) were tested to find optimal cell ratio for efficient differentiation of ASCs by histochemical staining, cell proliferation, gene expression and so on. According RT-PCR and staining, differentiation of ASCs was dependent on the media condition and cell ratio between NP cells and ASCs. This demonstrated the appropriate media and the optimized cell ratio between NP cells and ASCs are able to induce efficient differentiation of ASCs that is expected to provide efficient intervertebral disc regeneration in vivo.
Original language | English |
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Pages (from-to) | 461-466 |
Number of pages | 6 |
Journal | Tissue Engineering and Regenerative Medicine |
Volume | 6 |
Issue number | 4-11 |
State | Published - Jun 2009 |
Keywords
- ASC
- Co-culture
- Differentiation
- Nucleus pulposus cells