Co-culture condition of adipose derived stromal cells and nucleus pulposus cells for regeneration of intervertebral disc

Eun Hee Choi, Kwang Sook Park, In Bo Han, Sun Woong Kang, Soo Hong Lee

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Nucleus pulposus(NP) cells located at intervertebral disc are chondrocyte-like cells secreting cartilagespecific matrix molecules such as type 2 collagen and aggrecan. NP cells transplantation is used for recovery of damaged intervertebral disc. However, it is difficult to obtain sufficient quantity of NP cells from donor for cell therapy. Recently, adipose derived stromal cells(ASCs) as alternative cell sources have been extensively studied for cells therapy because ASCs can differentiate into several lineages including chondrogenesis. In this study, we sought to optimize the co-culture condition of NP cells and human ASCs for ASC differentiation. Media condition for NP cells and ASCs co-culture was determined by comparing proliferation and differentiation. After that, various cell ratios between NP cells and ASCs(0, 25, 50, 75, 100% ASC) were tested to find optimal cell ratio for efficient differentiation of ASCs by histochemical staining, cell proliferation, gene expression and so on. According RT-PCR and staining, differentiation of ASCs was dependent on the media condition and cell ratio between NP cells and ASCs. This demonstrated the appropriate media and the optimized cell ratio between NP cells and ASCs are able to induce efficient differentiation of ASCs that is expected to provide efficient intervertebral disc regeneration in vivo.

Original languageEnglish
Pages (from-to)461-466
Number of pages6
JournalTissue Engineering and Regenerative Medicine
Volume6
Issue number4-11
StatePublished - Jun 2009

Keywords

  • ASC
  • Co-culture
  • Differentiation
  • Nucleus pulposus cells

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