TY - JOUR
T1 - Combination of transforming growth factor-beta2 and bone morphogenetic protein 7 enhances chondrogenesis from adipose tissue-derived mesenchymal stem cells
AU - Kim, Hye Joung
AU - Im, Gun Il
PY - 2009/7/1
Y1 - 2009/7/1
N2 - In this study, the authors examined combinations of growth factors that induce effective chondrogenesis from adipose tissue-derived mesenchymal stem cells (MSCs). Human MSCs were isolated from bone marrow (BMMSCs) and adipose tissue (ATMSCs) and characterized according to flow cytometry for CD34, CD45, CD73, and CD166. Chondrogenesis was induced by culturing ATMSCs in pellets without growth factors (negative control) and with 5ng/mL of transforming growth factor beta 2 (TGF-β2), 100ng/mL of bone morphogenetic protein (BMP)-2, 100ng/mL of BMP-6, 100ng/mL of BMP-7, 5ng/mL of TGF-β2 and 100ng/mL of BMP-2, 5ng/mL of TGF-β2 and 100ng/mL of BMP-6, and 5ng/mL of TGF-β2 and 100ng/mL of BMP-7. BMMSCs cultured under the same condition with 5ng/mL of TGF-β2 were used as positive controls. Flow cytometry showed that ATMSCs and BMMSCs had similar surface marker profiles. After 4 weeks of in vitro culture, glycosaminoglycan assays, real-time reverse transcriptase polymerase chain reaction, and histological findings demonstrated that the combination of 5ng/mL of TGF-β2 and 100ng/mL of BMP-7 most effectively induced chondrogenesis from ATMSCs. The findings of this study suggest that the combination of TGF-β2 and BMP-7 potently enhances chondrogenesis from ATMSCs and can be used to overcome the inferior chondrogenic potential of ATMSCs in cartilage tissue engineering.
AB - In this study, the authors examined combinations of growth factors that induce effective chondrogenesis from adipose tissue-derived mesenchymal stem cells (MSCs). Human MSCs were isolated from bone marrow (BMMSCs) and adipose tissue (ATMSCs) and characterized according to flow cytometry for CD34, CD45, CD73, and CD166. Chondrogenesis was induced by culturing ATMSCs in pellets without growth factors (negative control) and with 5ng/mL of transforming growth factor beta 2 (TGF-β2), 100ng/mL of bone morphogenetic protein (BMP)-2, 100ng/mL of BMP-6, 100ng/mL of BMP-7, 5ng/mL of TGF-β2 and 100ng/mL of BMP-2, 5ng/mL of TGF-β2 and 100ng/mL of BMP-6, and 5ng/mL of TGF-β2 and 100ng/mL of BMP-7. BMMSCs cultured under the same condition with 5ng/mL of TGF-β2 were used as positive controls. Flow cytometry showed that ATMSCs and BMMSCs had similar surface marker profiles. After 4 weeks of in vitro culture, glycosaminoglycan assays, real-time reverse transcriptase polymerase chain reaction, and histological findings demonstrated that the combination of 5ng/mL of TGF-β2 and 100ng/mL of BMP-7 most effectively induced chondrogenesis from ATMSCs. The findings of this study suggest that the combination of TGF-β2 and BMP-7 potently enhances chondrogenesis from ATMSCs and can be used to overcome the inferior chondrogenic potential of ATMSCs in cartilage tissue engineering.
UR - http://www.scopus.com/inward/record.url?scp=67650682082&partnerID=8YFLogxK
U2 - 10.1089/ten.tea.2008.0368
DO - 10.1089/ten.tea.2008.0368
M3 - Article
AN - SCOPUS:67650682082
SN - 1937-3341
VL - 15
SP - 1543
EP - 1551
JO - Tissue Engineering - Part A.
JF - Tissue Engineering - Part A.
IS - 7
ER -