Abstract
Efficient cofactor regeneration is essential for the utilization of many oxidoreductases. Herein, the β-subunit of formate dehydrogenase 1 from Methylobacterium extorquens AM1 (MeFDH1-β) was employed as a novel electroactive biocatalyst for electroenzymatic NADH regeneration for the first time. MeFDH1-β showed much better properties in both catalytic activity (a turnover frequency of 3,600 hr−1) and current efficiency (ca. 98%) than a conventional rhodium (III)-based chemical catalyst (M = [Cp∗Rh(bpy)H2O]2+, Cp∗ = C5Me5, bpy = 2,2-bipyridine) and a traditional biocatalyst (diaphorase).
Original language | English |
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Pages (from-to) | G50-G52 |
Journal | Journal of the Electrochemical Society |
Volume | 163 |
Issue number | 5 |
DOIs | |
State | Published - 2016 |