Communication—highly efficient electroenzymatic nadh regeneration by an electron-relay flavoenzyme

Sumi Lee, Hyunjun Choe, Dae Haeng Cho, Sung Ho Yoon, Keehoon Won, Yong Hwan Kima

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Efficient cofactor regeneration is essential for the utilization of many oxidoreductases. Herein, the β-subunit of formate dehydrogenase 1 from Methylobacterium extorquens AM1 (MeFDH1-β) was employed as a novel electroactive biocatalyst for electroenzymatic NADH regeneration for the first time. MeFDH1-β showed much better properties in both catalytic activity (a turnover frequency of 3,600 hr−1) and current efficiency (ca. 98%) than a conventional rhodium (III)-based chemical catalyst (M = [CpRh(bpy)H2O]2+, Cp = C5Me5, bpy = 2,2-bipyridine) and a traditional biocatalyst (diaphorase).

Original languageEnglish
Pages (from-to)G50-G52
JournalJournal of the Electrochemical Society
Volume163
Issue number5
DOIs
StatePublished - 2016

Fingerprint

Dive into the research topics of 'Communication—highly efficient electroenzymatic nadh regeneration by an electron-relay flavoenzyme'. Together they form a unique fingerprint.

Cite this