Comparison of three sample preparation methods on the recovery of volatiles from taheebo (Tabebuia impetiginosa Martius ex DC)

Byeoung Soo Park, Kwang Geun Lee, Gary R. Takeoka

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

The volatile constituents of taheebo (the dried inner bark of Tabebuia impeliginosa Martius ex DC) were isolated by three methods: solvent-assisted flavour evaporation (SAFE); steam distillation under reduced pressure, followed by continuous liquid-liquid extraction (DRP-LLE); and high-flow dynamic headspace sampling (including closed-loop stripping) (DHS). The extracts were analysed qualitatively and quantitatively by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The masses of total volatile components recovered from 50 g taheebo by SAFE, DRP-LLE and DHS were 26.3 ± 2.0, 19.4 ± 1.0 and 1.2 ± 0.2 mg, respectively. The major constituents isolated with the SAFE method were 4-methoxyphenol (121.65 μg/g), 4-methoxybenzyl alcohol (96.49 μg/g), 1,2-propanediol (92.95 μg/g) and 4-methoxybenzaldehyde (31.33 μg/g). The DRP-LLC method yielded 4-methoxybenzaldehyde (64.54 μg/g), 4-methoxyphenol (42.30 μg/g), 5-(2-propenyl)-1,2,3-trimethoxybenzene (elemicin; 41.22 μg/g), and 1-methoxy-4-(1E)-1-propenylbenzene (trans-anethole; 39.16 μg/g) as major volatiles, while the main compounds recovered with the DHS method were 4-methoxybenzaldehyde (2.61 μg/g), 4-methoxyphenol (2.10 μg/g), 2-methyl-5-(1-methylethenyl)-2-cyclohexen-1-one (carvone; 1.71 μg/g) and 3,7-dimethyl-1,6-octadien-3-ol (linalool; 1.64 μg/g).

Original languageEnglish
Pages (from-to)287-292
Number of pages6
JournalFlavour and Fragrance Journal
Volume19
Issue number4
DOIs
StatePublished - Jul 2004

Keywords

  • Dynamic headspace sampling (DHS)
  • Pau d'arco
  • Solvent-assisted flavour evaporation (SAFE)
  • Volatile components

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