Concave microwell plate facilitates chondrogenesis from mesenchymal stem cells

Ji Yun Ko, Gun Il Im

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Objectives: To compare in vitro chondrogenesis from bone marrow-derived mesenchymal stem cells using concave microwell plates with those obtained using culture tubes. Results: Pellets cultured in concave microwell plates had a significantly higher level of GAG per DNA content and greater proteoglycan content than those cultured in tubes at day 7 and 14. Three chondrogenic markers, SOX-9, COL2A1 and aggrecan, showed significantly higher expression in pellets cultured in concave microwell plates than those cultured in tubes at day 7 and 14. At day 21, there was not a significant difference in the expression of these markers. COL10A1, the typical hypertrophy marker, was significantly lower in concave microwell plates during the whole culture period. Runx-2, a marker of hypertrophy and osteogenesis, was significantly lower at day 7 in pellets cultured in concave microwell plates than those cultured in tubes. Conclusion: Concave microwell plates provide a convenient and effective tool for the study of in vitro chondrogenesis and may replace the use of propylene culture tube.

Original languageEnglish
Pages (from-to)1967-1974
Number of pages8
JournalBiotechnology Letters
Volume38
Issue number11
DOIs
StatePublished - 1 Nov 2016

Keywords

  • Chondrogenesis
  • Chondrogenic markers
  • Concave microwell plate
  • Mesenchymal cells
  • Microwell culture
  • Pellet culture

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