Abstract
Objectives: To compare in vitro chondrogenesis from bone marrow-derived mesenchymal stem cells using concave microwell plates with those obtained using culture tubes. Results: Pellets cultured in concave microwell plates had a significantly higher level of GAG per DNA content and greater proteoglycan content than those cultured in tubes at day 7 and 14. Three chondrogenic markers, SOX-9, COL2A1 and aggrecan, showed significantly higher expression in pellets cultured in concave microwell plates than those cultured in tubes at day 7 and 14. At day 21, there was not a significant difference in the expression of these markers. COL10A1, the typical hypertrophy marker, was significantly lower in concave microwell plates during the whole culture period. Runx-2, a marker of hypertrophy and osteogenesis, was significantly lower at day 7 in pellets cultured in concave microwell plates than those cultured in tubes. Conclusion: Concave microwell plates provide a convenient and effective tool for the study of in vitro chondrogenesis and may replace the use of propylene culture tube.
Original language | English |
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Pages (from-to) | 1967-1974 |
Number of pages | 8 |
Journal | Biotechnology Letters |
Volume | 38 |
Issue number | 11 |
DOIs | |
State | Published - 1 Nov 2016 |
Keywords
- Chondrogenesis
- Chondrogenic markers
- Concave microwell plate
- Mesenchymal cells
- Microwell culture
- Pellet culture