Controlled assembly for well-defined 3D bioarchitecture using two active enzymes

Dong Chung Kim, Jung Inn Sohn, Dejian Zhou, Thomas A.J. Duke, Dae Joon Kang

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

This paper reports that a bioarchitecture with two different active enzymes can be fabricated conveniently on a prepatterned surface by highly selective surface-templated layer-by-layer (LBL) assembly by coupling a bilayer of avidin/biotin-lactate oxidase (biotin-LOD) with a bilayer of avidin/biotin-horseradish peroxidase (biotin-HRP). The two different active enzymes can be utilized as excellent building blocks for the fabrication of well-defined 3D nanostructures with precise control of the position and height on the surface. In addition, the LBL assembled bienzyme structures are highly functional, and bioarchitectures based on LOD and HRP-mediated coupling reaction can be employed in a number of viable biosensing applications.

Original languageEnglish
Pages (from-to)1580-1586
Number of pages7
JournalACS Nano
Volume4
Issue number3
DOIs
StatePublished - 23 Mar 2010

Keywords

  • Bioarchitecture
  • Horseradish peroxidase
  • Lactate oxidase
  • LBL assembly
  • Well-defined 3D nanostructure

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