Crystal structure of Pyrococcus furiosus PF2050, a member of the DUF2666 protein family

Byeong Gu Han; Kyung Chae Jeong; Jea Won Cho; Byung Cheon Jeong; Hyun Kyu Song; Jae Young Lee; Dong Hyuk Shin; Sangho Lee; Byung Il Lee

doi:10.1016/j.febslet.2012.04.004

Crystal structure of Pyrococcus furiosus PF2050, a member of the DUF2666 protein family

Byeong Gu Han, Kyung Chae Jeong, Jea Won Cho, Byung Cheon Jeong, Hyun Kyu Song, Jae Young Lee, Dong Hyuk Shin, Sangho Lee, Byung Il Lee

Department of Life Science

Research output: Contribution to journal › Article › peer-review

Abstract

Pyrococcus furiosus PF2050 is an uncharacterized putative protein that contains two DUF2666 domains. Functional and structural studies of PF2050 have not previously been performed. In this study, we determined the crystal structure of PF2050. The structure of PF2050 showed that the two DUF2666 domains interact tightly, forming a globular structure. Each DUF2666 domain comprises an antiparallel β-sheet and an α-helical bundle. One side of the PF2050 structure has a positively charged basic cleft, which may have a DNA-binding function. Furthermore, we confirmed that PF2050 interacts with circular and linear dsDNA.

Original language	English
Pages (from-to)	1384-1388
Number of pages	5
Journal	FEBS Letters
Volume	586
Issue number	9
DOIs	https://doi.org/10.1016/j.febslet.2012.04.004
State	Published - 7 May 2012

Keywords

DNA binding
DUF2666
PF2050
Pyrococcus furiosus

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10.1016/j.febslet.2012.04.004

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title = "Crystal structure of Pyrococcus furiosus PF2050, a member of the DUF2666 protein family",

abstract = "Pyrococcus furiosus PF2050 is an uncharacterized putative protein that contains two DUF2666 domains. Functional and structural studies of PF2050 have not previously been performed. In this study, we determined the crystal structure of PF2050. The structure of PF2050 showed that the two DUF2666 domains interact tightly, forming a globular structure. Each DUF2666 domain comprises an antiparallel β-sheet and an α-helical bundle. One side of the PF2050 structure has a positively charged basic cleft, which may have a DNA-binding function. Furthermore, we confirmed that PF2050 interacts with circular and linear dsDNA.",

keywords = "DNA binding, DUF2666, PF2050, Pyrococcus furiosus",

author = "Han, {Byeong Gu} and Jeong, {Kyung Chae} and Cho, {Jea Won} and Jeong, {Byung Cheon} and Song, {Hyun Kyu} and Lee, {Jae Young} and Shin, {Dong Hyuk} and Sangho Lee and Lee, {Byung Il}",

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month = may,

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doi = "10.1016/j.febslet.2012.04.004",

language = "English",

volume = "586",

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journal = "FEBS Letters",

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T1 - Crystal structure of Pyrococcus furiosus PF2050, a member of the DUF2666 protein family

AU - Han, Byeong Gu

AU - Jeong, Kyung Chae

AU - Cho, Jea Won

AU - Jeong, Byung Cheon

AU - Song, Hyun Kyu

AU - Lee, Jae Young

AU - Shin, Dong Hyuk

AU - Lee, Sangho

AU - Lee, Byung Il

PY - 2012/5/7

Y1 - 2012/5/7

N2 - Pyrococcus furiosus PF2050 is an uncharacterized putative protein that contains two DUF2666 domains. Functional and structural studies of PF2050 have not previously been performed. In this study, we determined the crystal structure of PF2050. The structure of PF2050 showed that the two DUF2666 domains interact tightly, forming a globular structure. Each DUF2666 domain comprises an antiparallel β-sheet and an α-helical bundle. One side of the PF2050 structure has a positively charged basic cleft, which may have a DNA-binding function. Furthermore, we confirmed that PF2050 interacts with circular and linear dsDNA.

AB - Pyrococcus furiosus PF2050 is an uncharacterized putative protein that contains two DUF2666 domains. Functional and structural studies of PF2050 have not previously been performed. In this study, we determined the crystal structure of PF2050. The structure of PF2050 showed that the two DUF2666 domains interact tightly, forming a globular structure. Each DUF2666 domain comprises an antiparallel β-sheet and an α-helical bundle. One side of the PF2050 structure has a positively charged basic cleft, which may have a DNA-binding function. Furthermore, we confirmed that PF2050 interacts with circular and linear dsDNA.

KW - DNA binding

KW - DUF2666

KW - PF2050

KW - Pyrococcus furiosus

UR - http://www.scopus.com/inward/record.url?scp=84860346744&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2012.04.004

DO - 10.1016/j.febslet.2012.04.004

M3 - Article

C2 - 22616997

AN - SCOPUS:84860346744

SN - 0014-5793

VL - 586

SP - 1384

EP - 1388

JO - FEBS Letters

JF - FEBS Letters

IS - 9

ER -

Crystal structure of Pyrococcus furiosus PF2050, a member of the DUF2666 protein family

Abstract

Keywords

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