TY - JOUR
T1 - Crystallization and preliminary X-ray crystallographic analysis of enoyl-acyl carrier protein reductase from Helicobacter pylori
AU - Hyung, Ho Lee
AU - Yun, Jungmin
AU - Moon, Jinho
AU - Byung, Woo Han
AU - Byung, Il Lee
AU - Jae, Young Lee
AU - Se, Won Suh
PY - 2002
Y1 - 2002
N2 - Enoyl-acyl carrier protein reductase (ENR) catalyzes the NADH-dependent stereospecific reduction of α,β-unsaturated fatty acids bound to the acyl-carrier protein. ENR from Helicobacter pylori has been overexpressed in Escherichia coli and has been crystallized in the presence of its cofactor NADH and the inhibitor triclosan (or its analogue diclosan) at 296 K using polyethylene glycol (PEG) 400 as a precipitant. For the triclosan (or diclosan) complex, diffraction data to 2.5 (or 2.3) Å resolution have been collected using synchrotron X-rays. The crystals belong to the monoclinic space group P21, with unit-cell parameters a = 73.35, b = 94.91, c = 75.38 Å, β = 106.21° for the triclosan complex (or a = 73.25, 5 = 95.07, c = 75.02 Å, β = 106.53° for the diclosan complex). The asymmetric unit contains one homotetramer, with a corresponding VM of 2.10 Å3 Da-1 and a solvent content of 41% by volume.
AB - Enoyl-acyl carrier protein reductase (ENR) catalyzes the NADH-dependent stereospecific reduction of α,β-unsaturated fatty acids bound to the acyl-carrier protein. ENR from Helicobacter pylori has been overexpressed in Escherichia coli and has been crystallized in the presence of its cofactor NADH and the inhibitor triclosan (or its analogue diclosan) at 296 K using polyethylene glycol (PEG) 400 as a precipitant. For the triclosan (or diclosan) complex, diffraction data to 2.5 (or 2.3) Å resolution have been collected using synchrotron X-rays. The crystals belong to the monoclinic space group P21, with unit-cell parameters a = 73.35, b = 94.91, c = 75.38 Å, β = 106.21° for the triclosan complex (or a = 73.25, 5 = 95.07, c = 75.02 Å, β = 106.53° for the diclosan complex). The asymmetric unit contains one homotetramer, with a corresponding VM of 2.10 Å3 Da-1 and a solvent content of 41% by volume.
UR - http://www.scopus.com/inward/record.url?scp=0036072010&partnerID=8YFLogxK
U2 - 10.1107/S0907444902007151
DO - 10.1107/S0907444902007151
M3 - Article
C2 - 12037321
AN - SCOPUS:0036072010
SN - 0907-4449
VL - 58
SP - 1071
EP - 1073
JO - Acta Crystallographica Section D: Biological Crystallography
JF - Acta Crystallographica Section D: Biological Crystallography
IS - 6 II
ER -