TY - JOUR
T1 - Crystallization and preliminary x-ray crystallographic analysis of type II dehydroquinase from Helicobacter pylori
AU - Kwak, J. E.
AU - Lee, J. Y.
AU - Han, B. W.
AU - Moon, J.
AU - Sohn, S. H.
AU - Suh, S. W.
PY - 2001
Y1 - 2001
N2 - The enzyme 3-dehydroquinase catalyzes the interconversion of 3-dehydroquinate and 3-dehydroshikimate. The enzymes are classified into two groups, type I and type II, which have different biochemical and biophysical properties and act with different mechanisms. The type II, dehydroquinase of Helicobacter pylori, a dodecameric enzyme, was overexpressed in Escherichia coli. The recombinant protein has been crystallized at 296 K using polyethylene glycol (PEG) 4000 as a precipitant. Native X-ray diffraction data have been collected to 2.5 Å resolution using synchrotron radiation. The crystals are cubic and belong to the space group P4232, with unit-cell parameters a = b = c = 98,91 Å. The asymmetric unit contains one subunit of recombinant type II dehydroquinase, with a corresponding VM of 2.18 Å3 Da-1 and a solvent content of 43.6%.
AB - The enzyme 3-dehydroquinase catalyzes the interconversion of 3-dehydroquinate and 3-dehydroshikimate. The enzymes are classified into two groups, type I and type II, which have different biochemical and biophysical properties and act with different mechanisms. The type II, dehydroquinase of Helicobacter pylori, a dodecameric enzyme, was overexpressed in Escherichia coli. The recombinant protein has been crystallized at 296 K using polyethylene glycol (PEG) 4000 as a precipitant. Native X-ray diffraction data have been collected to 2.5 Å resolution using synchrotron radiation. The crystals are cubic and belong to the space group P4232, with unit-cell parameters a = b = c = 98,91 Å. The asymmetric unit contains one subunit of recombinant type II dehydroquinase, with a corresponding VM of 2.18 Å3 Da-1 and a solvent content of 43.6%.
UR - http://www.scopus.com/inward/record.url?scp=0035128225&partnerID=8YFLogxK
U2 - 10.1107/S0907444900016267
DO - 10.1107/S0907444900016267
M3 - Article
C2 - 11173479
AN - SCOPUS:0035128225
SN - 0907-4449
VL - 57
SP - 279
EP - 280
JO - Acta Crystallographica Section D: Biological Crystallography
JF - Acta Crystallographica Section D: Biological Crystallography
IS - 2
ER -