Crystallization and preliminary X-ray crystallographic analysis of human nucleoside diphosphate kinase A

Kyeongsik Min; Sun Young Kim; Hyun Kyu Song; Changsoo Chang; Seung Je Cho; Jinho Moon; Jin Kuk Yang; Jae Young Lee; Kong Joo Lee; Se Won Suh

doi:10.1107/S0907444900002626

Crystallization and preliminary X-ray crystallographic analysis of human nucleoside diphosphate kinase A

Kyeongsik Min
, Sun Young Kim
, Hyun Kyu Song
, Changsoo Chang
, Seung Je Cho
, Jinho Moon
, Jin Kuk Yang
, Jae Young Lee
, Kong Joo Lee
, Se Won Suh

Department of Life Science

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Human nucleoside diphosphate kinase A catalyzes phosphoryl transfer and acts as a suppressor of metastasis. It has been crystallized using 2-methyl-2,4-pentanediol as a precipitant at 288 K. The crystal is monoclinic, belonging to the space group P2₁, with unit-cell parameters a = 74.21, b = 78.11, c = 82.29 Å, β = 101.33°. The asymmetric unit contains a homohexamer, with a corresponding crystal volume per protein mass (V(m)) of 2.27 Å³ Da^-1 and a solvent content of 46%. Native X-ray data to 2.15 Å resolution have been collected using synchrotron X-rays.

Original language	English
Pages (from-to)	504-505
Number of pages	2
Journal	Acta Crystallographica Section D: Biological Crystallography
Volume	56
Issue number	4
DOIs	https://doi.org/10.1107/S0907444900002626
State	Published - 2000

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title = "Crystallization and preliminary X-ray crystallographic analysis of human nucleoside diphosphate kinase A",

abstract = "Human nucleoside diphosphate kinase A catalyzes phosphoryl transfer and acts as a suppressor of metastasis. It has been crystallized using 2-methyl-2,4-pentanediol as a precipitant at 288 K. The crystal is monoclinic, belonging to the space group P21, with unit-cell parameters a = 74.21, b = 78.11, c = 82.29 {\AA}, β = 101.33°. The asymmetric unit contains a homohexamer, with a corresponding crystal volume per protein mass (V(m)) of 2.27 {\AA}3 Da-1 and a solvent content of 46\%. Native X-ray data to 2.15 {\AA} resolution have been collected using synchrotron X-rays.",

author = "Kyeongsik Min and Kim, \{Sun Young\} and Song, \{Hyun Kyu\} and Changsoo Chang and Cho, \{Seung Je\} and Jinho Moon and Yang, \{Jin Kuk\} and Lee, \{Jae Young\} and Lee, \{Kong Joo\} and Suh, \{Se Won\}",

year = "2000",

doi = "10.1107/S0907444900002626",

language = "English",

volume = "56",

pages = "504--505",

journal = "Acta Crystallographica Section D: Biological Crystallography",

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TY - JOUR

T1 - Crystallization and preliminary X-ray crystallographic analysis of human nucleoside diphosphate kinase A

AU - Min, Kyeongsik

AU - Kim, Sun Young

AU - Song, Hyun Kyu

AU - Chang, Changsoo

AU - Cho, Seung Je

AU - Moon, Jinho

AU - Yang, Jin Kuk

AU - Lee, Jae Young

AU - Lee, Kong Joo

AU - Suh, Se Won

PY - 2000

Y1 - 2000

N2 - Human nucleoside diphosphate kinase A catalyzes phosphoryl transfer and acts as a suppressor of metastasis. It has been crystallized using 2-methyl-2,4-pentanediol as a precipitant at 288 K. The crystal is monoclinic, belonging to the space group P21, with unit-cell parameters a = 74.21, b = 78.11, c = 82.29 Å, β = 101.33°. The asymmetric unit contains a homohexamer, with a corresponding crystal volume per protein mass (V(m)) of 2.27 Å3 Da-1 and a solvent content of 46%. Native X-ray data to 2.15 Å resolution have been collected using synchrotron X-rays.

AB - Human nucleoside diphosphate kinase A catalyzes phosphoryl transfer and acts as a suppressor of metastasis. It has been crystallized using 2-methyl-2,4-pentanediol as a precipitant at 288 K. The crystal is monoclinic, belonging to the space group P21, with unit-cell parameters a = 74.21, b = 78.11, c = 82.29 Å, β = 101.33°. The asymmetric unit contains a homohexamer, with a corresponding crystal volume per protein mass (V(m)) of 2.27 Å3 Da-1 and a solvent content of 46%. Native X-ray data to 2.15 Å resolution have been collected using synchrotron X-rays.

UR - https://www.scopus.com/pages/publications/12944284654

U2 - 10.1107/S0907444900002626

DO - 10.1107/S0907444900002626

M3 - Article

C2 - 10739934

AN - SCOPUS:12944284654

SN - 0907-4449

VL - 56

SP - 504

EP - 505

JO - Acta Crystallographica Section D: Biological Crystallography

JF - Acta Crystallographica Section D: Biological Crystallography

IS - 4

ER -

Crystallization and preliminary X-ray crystallographic analysis of human nucleoside diphosphate kinase A

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