Cyclic mechanical strain promotes transforming-growth-factor-β1- mediated cardiomyogenic marker expression in bone-marrow-derived mesenchymal stem cells in vitro

Suk Ho Bhang, So Jung Gwak, Tae Jin Lee, Sang Soo Kim, Ho Hyun Park, Moon Hyang Park, Dae Hee Lee, Soo Hong Lee, Byung Soo Kim

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Cardiomyocytes in the heart reside in mechanically dynamic environments, such as those subject to cyclic mechanical strain. TGF-β1 (transforming growth factor-β1) stimulates cardiomyogenic marker expression of BMMSCs (bone-marrow-derived mesenchymal stem cells). In the present study, we tested the hypothesis that cyclic mechanical strain promotes TGF-β1-mediated cardiomyogenic marker expression in BMMSCs in vitro. The mRNA expression of cardiac-specific genes was more up-regulated in BMMSCs cultured with a TGF-β1 supplement and subjected to cyclic strain for 1 week than in BMMSCs cultured statically with a TGF-β1 supplement. Immunocytochemical analyses and flow cytometric analysis showed that the proportions of cardiac troponin-I-positive cells and cardiac MHC (myosin heavy chain)-positive cells and the proportions of cells expressing tropomyosin respectively were increased to a greater extent by TGF-β1with cyclic strain than by TGF-β1 alone. These results showed that cyclic strain promotes TGF-β1-mediated cardiomyogenic marker expression in BMMSCs in vitro.

Original languageEnglish
Pages (from-to)191-197
Number of pages7
JournalBiotechnology and Applied Biochemistry
Volume55
Issue number4
DOIs
StatePublished - Apr 2010

Keywords

  • Bone-marrow-derived mesenchymal stem cell (BMMSC)
  • Cardiomyogenic marker expression
  • Cyclic mechanical strain
  • Transforming growth factor-β1 (TGF-β1)

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