Deciphering the CHEF-PET-ESIPT liaison mechanism in a Zn2+ chemosensor and its applications in cell imaging study

Atanu Jana, Bhriguram Das, Sushil K. Mandal, Subhabrata Mabhai, Anisur R. Khuda-Bukhsh, Satyajit Dey

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

Proper fusion of fluorescence mechanisms in a single fluorophore unit is highly desirable to obtain better sensitivity, as well as selectivity towards a particular metal ion. In this regard, a Zn2+ chemosensor exhibiting strong fluorescence through synergistic CHEF-PET-ESIPT fluorescence mechanism has not received substantial deployment. This type of chemosensor provides an ample avenue to detect metal ions with a large stokes shift and are useful in living cell imaging study. Herein, we report an unparalleled Zn2+ chemosensor (L) based on these three types of fluorescence mechanisms by condensing 2,6-diformyl-4-methylphenol and 2-hydrazinyl-4,6-dimethylpyrimidine. All the three types of mechanism were experimentally verified by UV-Vis, fluorescence and time resolved photoluminescence (TRPL) spectroscopic evaluation. The sensor L and Zn2+ formed a 1:1 ratio complex demonstrating a high binding constant value (Ka = 4.812 × 105 M-1) and L showed clear discrimination from the Cd2+ ion. The limit of detection (LOD) and limit of quantification (LOQ) were calculated to be 9.727 × 10-7 (M) and 3.24 × 10-6 (M), respectively. We have also shown that cell imaging can be performed using L without many cytotoxicity concerns.

Original languageEnglish
Pages (from-to)5976-5984
Number of pages9
JournalNew Journal of Chemistry
Volume40
Issue number7
DOIs
StatePublished - 2016

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