TY - JOUR
T1 - Decolorization and biodegradation of textile dye Navy blue HER by Trichosporon beigelii NCIM-3326
AU - Saratale, R. G.
AU - Saratale, G. D.
AU - Chang, J. S.
AU - Govindwar, S. P.
PY - 2009/7/30
Y1 - 2009/7/30
N2 - Navy blue HER was decolorized and degraded within 24 h by Trichosporon beigelii NCIM-3326 under static condition. In the present study, we investigated various physicochemical parameters such as agitation, temperature, pH, cell concentration, initial dye concentration and different carbon and nitrogen sources to achieve maximum dye degradation by T. beigelii. Sequentially, decolorization and decrease in the total organic carbon (TOC) of Navy blue HER by T. beigelii were measured. Among five strains T. beigelii gave the better performance on the decolorization of Navy blue HER along with a 95% TOC reduction within 24 h. A significant increase in the activities of NADH-DCIP (dichlorophenolindophenol) reductase and azoreductase in the cells obtained after complete decolorization presumably indicates involvement of these enzymes in decolorization process. UV-vis, TLC, HPLC and FTIR analysis of extracted products confirmed the biodegradation of Navy blue HER. Phytotoxicity study demonstrated no toxicity of the biodegraded products with respect to plants viz. Phaseolus mungo and Sorghum vulgare. In addition to Navy blue HER, this strain also shows ability to decolorize various industrial dyes, including Red HE7B, Golden yellow 4BD, Green HE4BD, Orange HE2R, Malachite green, Crystal violet and Methyl violet.
AB - Navy blue HER was decolorized and degraded within 24 h by Trichosporon beigelii NCIM-3326 under static condition. In the present study, we investigated various physicochemical parameters such as agitation, temperature, pH, cell concentration, initial dye concentration and different carbon and nitrogen sources to achieve maximum dye degradation by T. beigelii. Sequentially, decolorization and decrease in the total organic carbon (TOC) of Navy blue HER by T. beigelii were measured. Among five strains T. beigelii gave the better performance on the decolorization of Navy blue HER along with a 95% TOC reduction within 24 h. A significant increase in the activities of NADH-DCIP (dichlorophenolindophenol) reductase and azoreductase in the cells obtained after complete decolorization presumably indicates involvement of these enzymes in decolorization process. UV-vis, TLC, HPLC and FTIR analysis of extracted products confirmed the biodegradation of Navy blue HER. Phytotoxicity study demonstrated no toxicity of the biodegraded products with respect to plants viz. Phaseolus mungo and Sorghum vulgare. In addition to Navy blue HER, this strain also shows ability to decolorize various industrial dyes, including Red HE7B, Golden yellow 4BD, Green HE4BD, Orange HE2R, Malachite green, Crystal violet and Methyl violet.
KW - Microbial dye decolorization
KW - Navy blue HER
KW - Phytotoxicity
KW - TOC
KW - Trichosporon beigelii
UR - http://www.scopus.com/inward/record.url?scp=67349211733&partnerID=8YFLogxK
U2 - 10.1016/j.jhazmat.2008.12.068
DO - 10.1016/j.jhazmat.2008.12.068
M3 - Article
C2 - 19157708
AN - SCOPUS:67349211733
SN - 0304-3894
VL - 166
SP - 1421
EP - 1428
JO - Journal of Hazardous Materials
JF - Journal of Hazardous Materials
IS - 2-3
ER -