Degradation and decolourization potential of an ligninolytic enzyme producing Aeromonas hydrophila for crystal violet dye and its phytotoxicity evaluation

Ram Naresh Bharagava, Sujata Mani, Sikandar I. Mulla, Ganesh Dattatraya Saratale

Research output: Contribution to journalArticlepeer-review

123 Scopus citations

Abstract

This study deals the biodegradation of crystal violet dye by a ligninolytic enzyme producing bacterium isolated from textile wastewater that was characterized and identified as Aeromonas hydrophila based on the 16 S rRNA gene sequence analysis. The degradation of crystal violet dye was studied under different environmental and nutritional conditions, and results showed that the isolated bacterium was effective to decolourize 99% crystal violet dye at pH 7 and temperature 35 °C in presence of sucrose and yeast extract as C and N source, respectively. This bacterium also produced lignin peroxidase and laccase enzyme, which were characterized by the SDS-PAGE analysis and found to have the molecular weight of ~ 40 and ~ 60 kDa, respectively. Further, the GC-MS analysis showed that CV dye was biotransformed into phenol, 2, 6-bis (1,1-dimethylethyl), 2′,6′-dihydroxyacetophenone and benzene by the isolated bacterium and the toxicity of CV dye was reduced upto a significant level as it showed 60%, 56.67% and 46.67% inhibition in seed germination. But, after the bacterial degradation/decolourization, it showed only 43.33%, 36.67% and 16.67% inhibition in seed germination after 24, 48 and 72 h, respectively. Thus, this study concluded that the isolated bacterium has high potential for the degradation/decolourization of CV dye as well to reduce its toxicity upto a significant level.

Original languageEnglish
Pages (from-to)166-175
Number of pages10
JournalEcotoxicology and Environmental Safety
Volume156
DOIs
StatePublished - 30 Jul 2018

Keywords

  • Aeromonas hydrophila
  • Biodegradation
  • Crystal violet
  • GC-MS analysis
  • Ligninolytic enzyme
  • Phytotoxicity

Fingerprint

Dive into the research topics of 'Degradation and decolourization potential of an ligninolytic enzyme producing Aeromonas hydrophila for crystal violet dye and its phytotoxicity evaluation'. Together they form a unique fingerprint.

Cite this