Deinococcus soli sp. Nov., a gamma-radiation-resistant bacterium isolated from rice field soil

Seho Cha, Sathiyaraj Srinivasan, Taegun Seo, Myung Kyum Kim

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24 Scopus citations

Abstract

A Gram-negative, non-motile, short rod-shaped bacterial strain, designated N5T, was isolated from a rice field soil in South Korea. Phylogenetic analysis based on the 16S rRNA gene sequence of the new isolate showed that strain N5T belongs to the genus Deinococcus, family Deinococcaceae, showing the highest sequence similarity to Deinococcus grandis KACC 11979 T (98.4 %) and Deinococcus daejeonensis KCTC 13751T (97.5 %). Strain N5T exhibits resistance to gamma-radiation similar to that of other members of the genus Deinococcus, with a D10 value in excess of 4 kGy. Chemotaxonomic data showed that the most abundant fatty acids are C16:1 ω7c (25.25 %), C15:1 ω6c (19.77 %), C17:1 ω6c (11.87 %), and C17:0 (9.41 %), and the major polar lipid is an unknown phosphoglycolipid. The predominant respiratory quinone is menaquinone MK-8. The DNA G+C content is 71.4 mol%. Phenotypic, phylogenetic, and chemotaxonomic data support designation of strain N5 T as a novel species of the genus Deinococcus, for which the name Deinococcus soli sp. Nov. is proposed. The type strain is N5T (=KCTC 33153T = JCM 19176T).

Original languageEnglish
Pages (from-to)777-783
Number of pages7
JournalCurrent Microbiology
Volume68
Issue number6
DOIs
StatePublished - Jun 2014

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