Design and development of caffeic acid conjugated with: Bombyx mori derived peptide biomaterials for anti-aging skin care applications

Over the last few centuries, aging-related diseases have become a part of the global health care problem. The skin is essentially more sensitive to aging because it is influenced by environmental exposure and genetic (genetically determined) processes. The use of a suitable cosmetic anti-aging material using a biomolecule with conventionally important medicine can help to protect damaged skin tissue. A new topical cosmetic anti-aging material should simulate the function of an extracellular matrix with its enhanced physiochemical, biological oxidative stress, and antioxidant properties. This study examined the significant features of CA-APPPKK (caffeic acid conjugated with peptide (APPPKK)) to determine if it meets the requirements for a cosmetic anti-aging biomolecule. Non-toxic CA-APPPKK was synthesized using a simple approach and CA stabilized with a peptide to form the new biomaterials. The cytotoxicity of the CA-APPPKK was examined with a CCK-8 assay and a reactive oxygen species (ROS) assay to evaluate the biological applications. CA-APPPKK caused a decrease in cell viability and membrane damage, and the degree of induction was dose dependent. The longevity-extending role of CA-APPPKK was attributed to its anti-oxidative activity and increased oxidative stress resistance. The anti-inflammatory capacity of CA-APPPKK was measured using a NO assay. This new approach indicates that CA-APPPKK is a potentially cosmetic anti-aging biomaterial for the prevention of oxidative stress-induced premature skin ageing and can be used to study aging-related diseases.