Detection of HIV-1 antigen by quartz crystal microbalance using gold nanoparticles

Tan Nhiem Ly; Sangkwon Park; Sang Joon Park

doi:10.1016/j.snb.2016.06.112

Detection of HIV-1 antigen by quartz crystal microbalance using gold nanoparticles

Tan Nhiem Ly, Sangkwon Park, Sang Joon Park

Department of Chemical and Biochemical Engineering

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

In the current exploratory study, a quartz crystal microbalance (QCM) technique was employed to detect HIV-1 antigen at a very low concentration using gold nanoparticles (Au NPs) as a signal enhancer. Colloidal Au NPs were synthesized using a reduction method followed by streptavidin functionalization. HIV-1 antigen was captured by a polyclonal antibody on the QCM surface that had been previously treated with 11-mercaptoundecanoicacid (MUA) and streptavidin. The Au NPs with a high mass amplified the detection signal, making HIV-1 antigen detection possible even in the window period of HIV infection. In addition, the effect of Au NP size was also investigated to elucidate the optimal size of the enhancer. The signal performance is discussed in terms of mass effect and conjugate efficiency.

Original language	English
Pages (from-to)	452-458
Number of pages	7
Journal	Sensors and Actuators B: Chemical
Volume	237
DOIs	https://doi.org/10.1016/j.snb.2016.06.112
State	Published - 1 Dec 2016

Keywords

Early detection
Gold nanoparticles
HIV-1 antigen
Quartz crystal microbalance
Signal amplification

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.snb.2016.06.112

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abstract = "In the current exploratory study, a quartz crystal microbalance (QCM) technique was employed to detect HIV-1 antigen at a very low concentration using gold nanoparticles (Au NPs) as a signal enhancer. Colloidal Au NPs were synthesized using a reduction method followed by streptavidin functionalization. HIV-1 antigen was captured by a polyclonal antibody on the QCM surface that had been previously treated with 11-mercaptoundecanoicacid (MUA) and streptavidin. The Au NPs with a high mass amplified the detection signal, making HIV-1 antigen detection possible even in the window period of HIV infection. In addition, the effect of Au NP size was also investigated to elucidate the optimal size of the enhancer. The signal performance is discussed in terms of mass effect and conjugate efficiency.",

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AU - Park, Sang Joon

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AB - In the current exploratory study, a quartz crystal microbalance (QCM) technique was employed to detect HIV-1 antigen at a very low concentration using gold nanoparticles (Au NPs) as a signal enhancer. Colloidal Au NPs were synthesized using a reduction method followed by streptavidin functionalization. HIV-1 antigen was captured by a polyclonal antibody on the QCM surface that had been previously treated with 11-mercaptoundecanoicacid (MUA) and streptavidin. The Au NPs with a high mass amplified the detection signal, making HIV-1 antigen detection possible even in the window period of HIV infection. In addition, the effect of Au NP size was also investigated to elucidate the optimal size of the enhancer. The signal performance is discussed in terms of mass effect and conjugate efficiency.

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Detection of HIV-1 antigen by quartz crystal microbalance using gold nanoparticles

Abstract

Keywords

UN SDGs

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