Development of chitosan-based cerium and titanium oxide loaded polycaprolactone for cutaneous wound healing and antibacterial applications

Anandhavelu Sanmugam, Logesh Kumar Sellappan, Swathy Manoharan, A. Rameshkumar, Raju Suresh Kumar, Abdulrahman I. Almansour, Natrajan Arumugam, Hyun Seok Kim, Dhanasekaran Vikraman

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Cerium dioxide (CeO2) based nanomaterials have emerged as promising dermal equivalents, promoting fibroblast infiltration and tissues regeneration. To enhance the antibacterial and wound healing activity, herein chitosan (CS)-CeO2 combined nano titanium dioxide (TiO2) complex loaded polycaprolactone (PCL) nanohybrid (CS-CeO2/TiO2/PCL) scaffolds were prepared through casting method. The nanohybrid scaffolds' physiochemical, morphological, mechanical, and biological properties were evaluated using advanced analytical techniques. Fourier transform infrared spectroscopy spectrum evidently depicted the various intermolecular interactions on the nanohybrid scaffolds. The developed scaffold exhibited the high swelling behavior and good degradability and permeability which is beneficial for absorbing wound transudation to fasten the healing efficacy. Moreover, CS-CeO2/TiO2/PCL scaffolds owned the better antibacterial activity against bacterial strains E. coli and S. aureus. Also, MTT assay on fibroblast (NIH 3T3) cells and immortalized human keratinocytes (HaCaT) cells indicated improved cell viability and proliferation. In vivo results revealed that the fabricated scaffold full aid to complete wound closure after 14 days which showed CS-CeO2/TiO2/PCL as the significant wound dressing material with potential antibacterial immunity.

Original languageEnglish
Article number128458
JournalInternational Journal of Biological Macromolecules
Volume256
DOIs
StatePublished - Jan 2024

Keywords

  • Antibacterial activity
  • TiO
  • Wound healing

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