TY - JOUR
T1 - Development of low cost upflow column bioreactor for degradation and detoxification of Blue HERD and textile effluent by Lysinibacillus sp. RGS immobilized on Loofa
AU - Bedekar, Priyanka A.
AU - Saratale, Rijuta G.
AU - Saratale, Ganesh D.
AU - Govindwar, Sanjay P.
N1 - Publisher Copyright:
© 2014 Elsevier Ltd.
PY - 2014/12/1
Y1 - 2014/12/1
N2 - Dyes and other toxic components of textile wastewater create severe hazards to receiving water. In the present study, Lysinibacillus sp. RGS, an effective dye degrading bacteria immobilized on Luffa cylindrica (Loofa) was used to decolorize sulphonated azo dye Blue HERD and 50% real textile effluent in an upflow column bioreactor. Blue HERD (200mgL-1) and 50% real textile effluent were degraded at volumetric flow rate 40mLh-1 and 15mLh-1, respectively. Induction in reductive (NADH-DCIP reductase and azoreductase) as well as oxidative (laccase, tyrosinase and veratryl alcohol oxidase) enzymes proved enzymatic decolorization of Blue HERD and 50% textile effluent. Significant reduction in COD, BOD, TOC, hardness and alkalinity of decolorized samples of Blue HERD and 50% effluent confirmed mineralization. HPTLC, FTIR and GCMS analysis confirmed degradation of Blue HERD into simple intermediates during decolorization in bioreactor. Less toxic nature of metabolites was confirmed using genotoxicity, cytotoxicity and phytotoxicity tests.
AB - Dyes and other toxic components of textile wastewater create severe hazards to receiving water. In the present study, Lysinibacillus sp. RGS, an effective dye degrading bacteria immobilized on Luffa cylindrica (Loofa) was used to decolorize sulphonated azo dye Blue HERD and 50% real textile effluent in an upflow column bioreactor. Blue HERD (200mgL-1) and 50% real textile effluent were degraded at volumetric flow rate 40mLh-1 and 15mLh-1, respectively. Induction in reductive (NADH-DCIP reductase and azoreductase) as well as oxidative (laccase, tyrosinase and veratryl alcohol oxidase) enzymes proved enzymatic decolorization of Blue HERD and 50% textile effluent. Significant reduction in COD, BOD, TOC, hardness and alkalinity of decolorized samples of Blue HERD and 50% effluent confirmed mineralization. HPTLC, FTIR and GCMS analysis confirmed degradation of Blue HERD into simple intermediates during decolorization in bioreactor. Less toxic nature of metabolites was confirmed using genotoxicity, cytotoxicity and phytotoxicity tests.
KW - Blue HERD
KW - Cytotoxicity
KW - Decolorization
KW - Genotoxicity
KW - Lysinibacillus sp. RGS
KW - Textile industry effluent
UR - http://www.scopus.com/inward/record.url?scp=84910059119&partnerID=8YFLogxK
U2 - 10.1016/j.ibiod.2014.09.007
DO - 10.1016/j.ibiod.2014.09.007
M3 - Article
AN - SCOPUS:84910059119
SN - 0964-8305
VL - 96
SP - 112
EP - 120
JO - International Biodeterioration and Biodegradation
JF - International Biodeterioration and Biodegradation
ER -