Development of real-time PCR for the detection of Clostridium perfringens in meats and vegetables

Jung Whan Chon, Jong Seok Park, Ji Yeon Hyeon, Chankyu Park, Kwang Young Song, Kwang Won Hong, In Gyun Hwang, Hyo Sun Kwak, Kun Ho Seo

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

A real-time PCR assay was developed and validated inhouse specifically for the detection of Clostridium perfringens (Cl. perfringens) in meats and vegetables by comparing with the culture method. The detection limit of the real-time PCR assay in phosphate-buffered saline was 102 CFU/ml. When the two methods were compared in food samples inoculated with Cl. perfringens, the culture method detected 52 positives, whereas real-time PCR detected 51 positives out of 160 samples. The difference was without statistical significance (p>0.05). Real-time PCR assay is an option for quality assurance laboratories to perform standard diagnostic tests, considering its detection ability and time-saving efficiency.

Original languageEnglish
Pages (from-to)530-534
Number of pages5
JournalJournal of Microbiology and Biotechnology
Volume22
Issue number4
DOIs
StatePublished - Apr 2012

Keywords

  • Clostridium perfringens
  • Culture method
  • Detection
  • Foods
  • Real-time PCR

Fingerprint

Dive into the research topics of 'Development of real-time PCR for the detection of Clostridium perfringens in meats and vegetables'. Together they form a unique fingerprint.

Cite this