TY - JOUR
T1 - Development of ultrasound aided chemical pretreatment methods to enrich saccharification of wheat waste biomass for polyhydroxybutyrate production and its characterization
AU - Saratale, Ganesh Dattatraya
AU - Saratale, Rijuta Ganesh
AU - Varjani, Sunita
AU - Cho, Si Kyung
AU - Ghodake, Gajanan S.
AU - Kadam, Avinash
AU - Mulla, Sikandar I.
AU - Bharagava, Ram Naresh
AU - Kim, Dong Su
AU - Shin, Han Seung
N1 - Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2020/8
Y1 - 2020/8
N2 - Development of an effective pretreatment method is a vital stage to enhance biomass-based biopolymers production. The present study aims on the pretreatment of wheat waste biomass (WS) with alkaline and in combination with ultrasound (US) treatment to enhance enzymatic saccharification of WS into monomeric sugars. Effects of chemical dosage, substrate loading, US power and US pretreatment time on the pretreatment efficiency were systematically investigated. Under optimized conditions; US + NaOH pretreatment attained maximum 70% delignification of WS, with 84.5% hydrolysis yield, 90% glucose yield and 65% xylose yield after enzymatic hydrolysis (30 FPU/g of dry WS) which is substantially higher than the individual pretreatment. Analytical studies (XRD, FTIR, and SEM) revealed that the combined US + NaOH pretreatment can effectively destroyed the ultrastructure of biomass and improved the accessibility to hydrolyzing enzymes. The resulted enzymatic hydrolysates were utilized as a possible biomass feedstock for polyhydroxybutyrate (PHB) production using Ralstonia eutropha. Effects of supplementation of nutrients, stressing agents and initial concentration of US + NaOH pretreated WS hydrolysates (20 to 40 g/L) on PHB production were evaluated. The maximum 74% PHA accumulation, PHB titer of 7.85 g/L and yield of PHB about 0.441 g/g of reducing sugar production was obtained. Analytical characterization and thermal characteristics of extracted biopolymer showed the poperies of standard PHB. The foregoing results suggested the potential of WS as an alternate renewable resource for sustainable biopolymer production and enables WS waste biomass disposal concerns.
AB - Development of an effective pretreatment method is a vital stage to enhance biomass-based biopolymers production. The present study aims on the pretreatment of wheat waste biomass (WS) with alkaline and in combination with ultrasound (US) treatment to enhance enzymatic saccharification of WS into monomeric sugars. Effects of chemical dosage, substrate loading, US power and US pretreatment time on the pretreatment efficiency were systematically investigated. Under optimized conditions; US + NaOH pretreatment attained maximum 70% delignification of WS, with 84.5% hydrolysis yield, 90% glucose yield and 65% xylose yield after enzymatic hydrolysis (30 FPU/g of dry WS) which is substantially higher than the individual pretreatment. Analytical studies (XRD, FTIR, and SEM) revealed that the combined US + NaOH pretreatment can effectively destroyed the ultrastructure of biomass and improved the accessibility to hydrolyzing enzymes. The resulted enzymatic hydrolysates were utilized as a possible biomass feedstock for polyhydroxybutyrate (PHB) production using Ralstonia eutropha. Effects of supplementation of nutrients, stressing agents and initial concentration of US + NaOH pretreated WS hydrolysates (20 to 40 g/L) on PHB production were evaluated. The maximum 74% PHA accumulation, PHB titer of 7.85 g/L and yield of PHB about 0.441 g/g of reducing sugar production was obtained. Analytical characterization and thermal characteristics of extracted biopolymer showed the poperies of standard PHB. The foregoing results suggested the potential of WS as an alternate renewable resource for sustainable biopolymer production and enables WS waste biomass disposal concerns.
KW - Biomass structure
KW - Enzymatic hydrolysis
KW - Poly(3-hydroxybutyrate)
KW - Ralstonia eutropha (ATCC 17699)
KW - Ultrasound pretreatment
KW - Wheat waste biomass
UR - http://www.scopus.com/inward/record.url?scp=85083345334&partnerID=8YFLogxK
U2 - 10.1016/j.indcrop.2020.112425
DO - 10.1016/j.indcrop.2020.112425
M3 - Article
AN - SCOPUS:85083345334
SN - 0926-6690
VL - 150
JO - Industrial Crops and Products
JF - Industrial Crops and Products
M1 - 112425
ER -