Discovery of hepatitis C virus NS3 helicase inhibitors by a multiplexed, high-throughput helicase activity assay based on graphene oxide

Hongje Jang, Soo Ryoon Ryoo, Young Kwan Kim, Soojin Yoon, Henna Kim, Sang Woo Han, Byong Seok Choi, Dong Eun Kim, Dal Hee Min

Research output: Contribution to journalArticlepeer-review

63 Scopus citations

Abstract

A GO-to solution: A simple graphene oxide (GO)-based assay to screen for selective inhibitors of a hepatitis C virus (HCV) helicase along with inhibitors of a severe acute respiratory syndrome coronavirus (SARS CoV) helicase was tested (see scheme). A single screen found five inhibitors highly selective for the HCV helicase orthologous to the SARS CoV helicase. Some of these hits were validated using the same GO-based assay.

Original languageEnglish
Pages (from-to)2340-2344
Number of pages5
JournalAngewandte Chemie - International Edition
Volume52
Issue number8
DOIs
StatePublished - 18 Feb 2013

Keywords

  • biosensors
  • graphene oxide
  • helicases
  • high-throughput screening
  • multiplex assays

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