Discovery of hepatitis C virus NS3 helicase inhibitors by a multiplexed, high-throughput helicase activity assay based on graphene oxide

Hongje Jang; Soo Ryoon Ryoo; Young Kwan Kim; Soojin Yoon; Henna Kim; Sang Woo Han; Byong Seok Choi; Dong Eun Kim; Dal Hee Min

doi:10.1002/anie.201209222

Discovery of hepatitis C virus NS3 helicase inhibitors by a multiplexed, high-throughput helicase activity assay based on graphene oxide

Hongje Jang
, Soo Ryoon Ryoo
, Young Kwan Kim
, Soojin Yoon
, Henna Kim
, Sang Woo Han
, Byong Seok Choi
, Dong Eun Kim
, Dal Hee Min

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

64 Scopus citations

Abstract

A GO-to solution: A simple graphene oxide (GO)-based assay to screen for selective inhibitors of a hepatitis C virus (HCV) helicase along with inhibitors of a severe acute respiratory syndrome coronavirus (SARS CoV) helicase was tested (see scheme). A single screen found five inhibitors highly selective for the HCV helicase orthologous to the SARS CoV helicase. Some of these hits were validated using the same GO-based assay.

Original language	English
Pages (from-to)	2340-2344
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	52
Issue number	8
DOIs	https://doi.org/10.1002/anie.201209222
State	Published - 18 Feb 2013

Keywords

biosensors
graphene oxide
helicases
high-throughput screening
multiplex assays

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/anie.201209222

Cite this

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title = "Discovery of hepatitis C virus NS3 helicase inhibitors by a multiplexed, high-throughput helicase activity assay based on graphene oxide",

abstract = "A GO-to solution: A simple graphene oxide (GO)-based assay to screen for selective inhibitors of a hepatitis C virus (HCV) helicase along with inhibitors of a severe acute respiratory syndrome coronavirus (SARS CoV) helicase was tested (see scheme). A single screen found five inhibitors highly selective for the HCV helicase orthologous to the SARS CoV helicase. Some of these hits were validated using the same GO-based assay.",

keywords = "biosensors, graphene oxide, helicases, high-throughput screening, multiplex assays",

author = "Hongje Jang and Ryoo, \{Soo Ryoon\} and Kim, \{Young Kwan\} and Soojin Yoon and Henna Kim and Han, \{Sang Woo\} and Choi, \{Byong Seok\} and Kim, \{Dong Eun\} and Min, \{Dal Hee\}",

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doi = "10.1002/anie.201209222",

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journal = "Angewandte Chemie - International Edition",

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AU - Jang, Hongje

AU - Ryoo, Soo Ryoon

AU - Kim, Young Kwan

AU - Yoon, Soojin

AU - Kim, Henna

AU - Han, Sang Woo

AU - Choi, Byong Seok

AU - Kim, Dong Eun

AU - Min, Dal Hee

PY - 2013/2/18

Y1 - 2013/2/18

N2 - A GO-to solution: A simple graphene oxide (GO)-based assay to screen for selective inhibitors of a hepatitis C virus (HCV) helicase along with inhibitors of a severe acute respiratory syndrome coronavirus (SARS CoV) helicase was tested (see scheme). A single screen found five inhibitors highly selective for the HCV helicase orthologous to the SARS CoV helicase. Some of these hits were validated using the same GO-based assay.

AB - A GO-to solution: A simple graphene oxide (GO)-based assay to screen for selective inhibitors of a hepatitis C virus (HCV) helicase along with inhibitors of a severe acute respiratory syndrome coronavirus (SARS CoV) helicase was tested (see scheme). A single screen found five inhibitors highly selective for the HCV helicase orthologous to the SARS CoV helicase. Some of these hits were validated using the same GO-based assay.

KW - biosensors

KW - graphene oxide

KW - helicases

KW - high-throughput screening

KW - multiplex assays

UR - https://www.scopus.com/pages/publications/84873923411

U2 - 10.1002/anie.201209222

DO - 10.1002/anie.201209222

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JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 8

ER -

Discovery of hepatitis C virus NS3 helicase inhibitors by a multiplexed, high-throughput helicase activity assay based on graphene oxide

Abstract

Keywords

UN SDGs

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