TY - JOUR
T1 - Discovery of potent and selective cytotoxic activity of new quinazoline-ureas against TMZ-resistant glioblastoma multiforme (GBM)
AU - Elkamhawy, Ahmed
AU - Viswanath, Ambily Nath Indu
AU - Pae, Ae Nim
AU - Kim, Hyeon Young
AU - Heo, Jin Chul
AU - Park, Woo Kyu
AU - Lee, Chong Ock
AU - Yang, Heekyoung
AU - Kim, Kang Ho
AU - Nam, Do Hyun
AU - Seol, Ho Jun
AU - Cho, Heeyeong
AU - Roh, Eun Joo
N1 - Publisher Copyright:
© 2015 Elsevier Masson SAS.
PY - 2015/10/20
Y1 - 2015/10/20
N2 - Herein, we report new quinazoline-urea based compounds with potent cytotoxic activities against TMZ-resistant glioblastoma multiforme (GBM) cells. Low micromolar IC50 values were exhibited over a panel of three primary GBM patient-derived cell cultures belonging to proneural (GBM-1), mesenchymal (GBM-2), and classical (GBM-3) subtypes. Eight compounds showed excellent selectivity indices for GBM cells comparing to a normal astrocyte cell line. In JC-1 assay, analogues 11, 12, 20, 22, and 24 exerted promising rates of mPTP opening induction towards proneural GBM subtype. Compounds 11, 20, and 24 bound to the translocator protein 18 kDa (TSPO) in submicromolar range using [3H] PK-11195 binding affinity assay. A homology model was built and docked models of 11, 12, 20, 22 and 24 were generated for describing their plausible binding modes in TSPO. In 3D clonogenic assay, compound 20 manifested potent tumoricidal effects on TMZ-resistant GBM cells even at submicromolar concentrations. In addition, CYP450 and hERG assays presented a safe toxicity profile of 20. Taken as a whole, this report presents compound 20 as a potent, selective and safe GBM cytotoxic agent which constitutes a promising direction against TMZ-resistant GBM.
AB - Herein, we report new quinazoline-urea based compounds with potent cytotoxic activities against TMZ-resistant glioblastoma multiforme (GBM) cells. Low micromolar IC50 values were exhibited over a panel of three primary GBM patient-derived cell cultures belonging to proneural (GBM-1), mesenchymal (GBM-2), and classical (GBM-3) subtypes. Eight compounds showed excellent selectivity indices for GBM cells comparing to a normal astrocyte cell line. In JC-1 assay, analogues 11, 12, 20, 22, and 24 exerted promising rates of mPTP opening induction towards proneural GBM subtype. Compounds 11, 20, and 24 bound to the translocator protein 18 kDa (TSPO) in submicromolar range using [3H] PK-11195 binding affinity assay. A homology model was built and docked models of 11, 12, 20, 22 and 24 were generated for describing their plausible binding modes in TSPO. In 3D clonogenic assay, compound 20 manifested potent tumoricidal effects on TMZ-resistant GBM cells even at submicromolar concentrations. In addition, CYP450 and hERG assays presented a safe toxicity profile of 20. Taken as a whole, this report presents compound 20 as a potent, selective and safe GBM cytotoxic agent which constitutes a promising direction against TMZ-resistant GBM.
KW - Clonogenic assay
KW - Docking
KW - Glioblastoma multiforme (GBM)
KW - Mitochondrial permeability transition pore (mPTP)
KW - Quinazoline-urea
KW - Toxicity profile
KW - Translocator protein 18 kDa (TSPO)
UR - http://www.scopus.com/inward/record.url?scp=84941135722&partnerID=8YFLogxK
U2 - 10.1016/j.ejmech.2015.08.001
DO - 10.1016/j.ejmech.2015.08.001
M3 - Article
C2 - 26355532
AN - SCOPUS:84941135722
SN - 0223-5234
VL - 103
SP - 210
EP - 222
JO - European Journal of Medicinal Chemistry
JF - European Journal of Medicinal Chemistry
ER -