DNA microarray analysis of gene expression profiles in Caenorhabditis elegans exposed to cadmium

Seok Won Jeong, Md Mujibur Rahman, Jeong Won Hwang, Jung Min Kim, Koji Arizono, Young Rok Seo

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The human population exposed to cadmium from air, food and water sources may exhibit abnormalities in multiple organs including the kidneys and the liver, and in the cardiovascular, immune and reproductive systems. In addition, the recognition of cadmium biomarkers which may increase cancer susceptibility are of prime importance. The investigation of the effects of cadmium exposure and the determination of its uptake using animal models are vital efforts that can be made towards identifying the health effects of cadmium exposure on humans. Caenorhabditis elegans (C. elegans) is a nematode commonly used as an animal model for genetic studies and a living biomonitor and which is useful for investigating basic biological processes such as mRNA expression in response to heavy metal exposure. In the present study, we investigated gene expression changes in C. elegans in response to cadmium chloride exposure using microarrays consisting of 22,000 nematode-oligonucleotide probes expressed (> 2 fold) following a 24 hour exposure to cadmium chloride. Several categories of genes have been detected from the microarray study and GO analysis. Based on their functions, they have been mapped according to cadmium ion responsive, we identified 958 genes, many of which are novel cadmium responsive genes, that were differentially angiogenesis and defense response to fungus. In conclusion, our investigation suggests importantecotoxociological biomarkers to be investigated in response to cadmium chloride exposure.

Original languageEnglish
Pages (from-to)35-41
Number of pages7
JournalBiochip Journal
Volume4
Issue number1
DOIs
StatePublished - 2010

Keywords

  • Biomarker
  • Cadmium chloride
  • Caenorhabditis elegans
  • Gene ontology (GO)
  • Microarray

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