Edaphocola aurantiacus gen. nov., sp. nov., a new member of the family Chitinophagaceae isolated from wetland soil in South Korea

A short rod-shaped, yellow–orange pigmented, strictly aerobic bacterium, designated as strain H2 ^T , was isolated from the wetland soil of Halla Mountain, Jeju-island, South Korea. Growth was observed at temperatures of 10–30 °C (optimum at 25–30 °C), pH of 6–8 (optimum at pH 7), and salt concentrations of 0–1% (w/v) NaCl (optimum at 0%). The strain H2 ^T was found to be a catalase and oxidase-positive, non-motile, Gram-negative bacterium. On the basis of 16S rRNA gene sequence similarity and phylogenetic analysis, strain H2 ^T was found to be related to the members of the Chitinophagaceae family, being closely related to Taibaiella chishuiensis AY17 ^T (94.3% sequence similarity). The major polar lipids are phosphatidylethanolamine and glycolipid. Strain H2 ^T contained MK-7 as the only menaquinone as well as iso-C _15:0 , iso-C _15:1 G and iso-C _17:0 3-OH as the major fatty acids (> 15%). The DNA G+C content of strain H2 ^T was determined to be 48.3 mol%. Based on the phylogenetic, phenotypic characteristics and chemotaxonomic analysis data, strain H2 ^T (= KCTC 62115 ^T = JCM 32353 ^T ) should be classified as representative of a novel species of a novel genus within the family Chitinophagaceae, for which the name Edaphocola aurantiacus gen. nov., sp. nov., is proposed.