Abstract
A MHDS has been employed to fabricate 3D scaffolds from PLGA with acetyl endgroups to achieve in vivo regeneration of cartilage tissue. The fabricated acetylated-PLGA scaffold showed open pores and interconnected structures. Rabbit chondrocytes were seeded on the PLGA scaffolds and transplanted immediately into subcutaneous sites of athymic mice. Chondrocytes transplantation with untreated PLGA scaffolds served as a control. Histological analysis of the implants at 4 weeks with H&E staining and alcian blue staining revealed higher extracellular matrix and GAG expression at the neocartilage in the PLGA-6Ac scaffolds than that of the PLGA-6OH scaffold group. This endgroup-modified scaffold may be useful for successful cartilage tissue engineering in orthopedic applications. Cartilage tissue engineering using polymer scaffolds could be an alternative option to treat cartilage injury. However, acidic conditions of the extracellular matrix has shown negative effects in chondrocytes. The acetylation of endgroup of PLGA would increase thermal stability. The increased thermal stability would be able to reduce degradation rate of biodegradable polymers and acidification of the extracellular matrix.
Original language | English |
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Pages (from-to) | 267-274 |
Number of pages | 8 |
Journal | Macromolecular Bioscience |
Volume | 11 |
Issue number | 2 |
DOIs | |
State | Published - 1 Feb 2011 |
Keywords
- Biodegradable
- Biological applications of polymers
- Biomaterials
- Degradation
- Tissue engineering