Abstract
The anticancer effect and apoptotic mechanism of a novel indole derivative AC-264, a lead derived from a chemical library, were investigated in human promyelocytic leukemia HL-60 cells. HL-60 cells treated with AC-264 at various concentrations showed the morphological features of apoptosis, such as plasma membrane blebbing and cell shrinkage. AC-264 exhibited cytotoxic effect in various cancer cell lines with different degrees of potency. Especially, AC-264 was effective on increasing the population of apoptotic cells in HL-60 cells, as detected by the number of cells stained with Annexin V and PI. Furthermore, AC-264 activated caspase-3 enzyme activity and induced internucleosomal DNA fragmentation. These results indicated that AC-264 produces anti-cancer effect via apoptotic cell death by activating caspase-3 and inducing internucleosomal DNA fragmentation in HL-60 cells.
Original language | English |
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Pages (from-to) | 3777-3781 |
Number of pages | 5 |
Journal | Bulletin of the Korean Chemical Society |
Volume | 31 |
Issue number | 12 |
DOIs | |
State | Published - 20 Dec 2010 |
Keywords
- Apoptosis
- Caspase-3
- DNA fragmentation
- Indole derivative