Effect of AC-264, a novel indole derivative, on apoptosis in HL-60 cells

Kyeong Lee, Ok Kyoung Kwon, Yan Xia, Kyung Seop Ahn

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The anticancer effect and apoptotic mechanism of a novel indole derivative AC-264, a lead derived from a chemical library, were investigated in human promyelocytic leukemia HL-60 cells. HL-60 cells treated with AC-264 at various concentrations showed the morphological features of apoptosis, such as plasma membrane blebbing and cell shrinkage. AC-264 exhibited cytotoxic effect in various cancer cell lines with different degrees of potency. Especially, AC-264 was effective on increasing the population of apoptotic cells in HL-60 cells, as detected by the number of cells stained with Annexin V and PI. Furthermore, AC-264 activated caspase-3 enzyme activity and induced internucleosomal DNA fragmentation. These results indicated that AC-264 produces anti-cancer effect via apoptotic cell death by activating caspase-3 and inducing internucleosomal DNA fragmentation in HL-60 cells.

Original languageEnglish
Pages (from-to)3777-3781
Number of pages5
JournalBulletin of the Korean Chemical Society
Volume31
Issue number12
DOIs
StatePublished - 20 Dec 2010

Keywords

  • Apoptosis
  • Caspase-3
  • DNA fragmentation
  • Indole derivative

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