Effect of inducers on the decolorization and biodegradation of textile azo dye Navy blue 2GL by Bacillus sp. VUS

Vishal V. Dawkar; Umesh U. Jadhav; Gajanan S. Ghodake; Sanjay P. Govindwar

doi:10.1007/s10532-009-9266-y

Effect of inducers on the decolorization and biodegradation of textile azo dye Navy blue 2GL by Bacillus sp. VUS

Vishal V. Dawkar, Umesh U. Jadhav, Gajanan S. Ghodake, Sanjay P. Govindwar

Department of Biological and Environmental Science

Shivaji University

Research output: Contribution to journal › Article › peer-review

100 Scopus citations

Abstract

Bacillus sp. VUS decolorized azo dye Navy blue 2GL in 48 h at static anoxic condition in yeast extract medium, whereas it took only 18 h for the decolorization in presence of CaCl₂. Different inducers played role in the decolorization of Navy blue 2GL. CaCl₂ found to be the most effective inducer among all inducers tested. The activity of enzymes like lignin peroxidase, laccase and reductases viz. NADH-DCIP, azo and riboflavin induced during decolorization represents their role in the biodegradation. Extracellular LiP and intracellular laccase activity induced with CaCl₂. Yeast extract was best medium for faster decolorization than other media. UV-vis spectrophotometer analysis and visual examinations showed decolorization of dye. High performance liquid chromatography, Fourier transforms infrared spectroscopy showed degradation of dye. Gas Chromatography-Mass Spectroscopy revealed formation of 4-Amino-3-(2-bromo-4, 6-dinitro-phenylazo)-phenol and acetic acid 2-(-acetoxy-ethylamino)-ethyl ester as final products. Bacillus sp. VUS also decolorized synthetic effluent. Phytotoxicity study showed detoxification of Navy blue 2GL.

Original language	English
Pages (from-to)	777-787
Number of pages	11
Journal	Biodegradation
Volume	20
Issue number	6
DOIs	https://doi.org/10.1007/s10532-009-9266-y
State	Published - Oct 2009

Keywords

Bacillus sp.
Biodegradation
GC-MS
Inducers
Lignin peroxidase
Navy blue 2GL

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title = "Effect of inducers on the decolorization and biodegradation of textile azo dye Navy blue 2GL by Bacillus sp. VUS",

abstract = "Bacillus sp. VUS decolorized azo dye Navy blue 2GL in 48 h at static anoxic condition in yeast extract medium, whereas it took only 18 h for the decolorization in presence of CaCl2. Different inducers played role in the decolorization of Navy blue 2GL. CaCl2 found to be the most effective inducer among all inducers tested. The activity of enzymes like lignin peroxidase, laccase and reductases viz. NADH-DCIP, azo and riboflavin induced during decolorization represents their role in the biodegradation. Extracellular LiP and intracellular laccase activity induced with CaCl2. Yeast extract was best medium for faster decolorization than other media. UV-vis spectrophotometer analysis and visual examinations showed decolorization of dye. High performance liquid chromatography, Fourier transforms infrared spectroscopy showed degradation of dye. Gas Chromatography-Mass Spectroscopy revealed formation of 4-Amino-3-(2-bromo-4, 6-dinitro-phenylazo)-phenol and acetic acid 2-(-acetoxy-ethylamino)-ethyl ester as final products. Bacillus sp. VUS also decolorized synthetic effluent. Phytotoxicity study showed detoxification of Navy blue 2GL.",

keywords = "Bacillus sp., Biodegradation, GC-MS, Inducers, Lignin peroxidase, Navy blue 2GL",

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AU - Dawkar, Vishal V.

AU - Jadhav, Umesh U.

AU - Ghodake, Gajanan S.

AU - Govindwar, Sanjay P.

PY - 2009/10

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N2 - Bacillus sp. VUS decolorized azo dye Navy blue 2GL in 48 h at static anoxic condition in yeast extract medium, whereas it took only 18 h for the decolorization in presence of CaCl2. Different inducers played role in the decolorization of Navy blue 2GL. CaCl2 found to be the most effective inducer among all inducers tested. The activity of enzymes like lignin peroxidase, laccase and reductases viz. NADH-DCIP, azo and riboflavin induced during decolorization represents their role in the biodegradation. Extracellular LiP and intracellular laccase activity induced with CaCl2. Yeast extract was best medium for faster decolorization than other media. UV-vis spectrophotometer analysis and visual examinations showed decolorization of dye. High performance liquid chromatography, Fourier transforms infrared spectroscopy showed degradation of dye. Gas Chromatography-Mass Spectroscopy revealed formation of 4-Amino-3-(2-bromo-4, 6-dinitro-phenylazo)-phenol and acetic acid 2-(-acetoxy-ethylamino)-ethyl ester as final products. Bacillus sp. VUS also decolorized synthetic effluent. Phytotoxicity study showed detoxification of Navy blue 2GL.

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Effect of inducers on the decolorization and biodegradation of textile azo dye Navy blue 2GL by Bacillus sp. VUS

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