Effect of low temperature on Schwann-like cell differentiation of bone marrow mesenchymal stem cells

Hee Hoon Yoon, Mi Jung Han, Jung Keug Park, Jong Ho Lee, Young Kwon Seo

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Mesenchymal stem cells (MSCs) are multipotent stromal cells that can differentiate into a variety of cell types. The potential of MSCs for differentiation and proliferation depends on the control of various processes. In this study, we have investigated the effects of low temperature on the Schwann-like cell differentiation of human bone marrow mesenchymal stem cells (BM-MSCs). The growth of BM-MSCs decreased at low temperature, but increased Schwann-like cell differentiation. The expression of cell surface antigen decreased CD73, CD105, and CD90, which are known markers of MSCs at 35°C. Also, the levels of matrix RNA [S100, glial fibrillary acidic protein (GFAP), p75, myelin basic protein, P0, and others] at 35°C in the differentiation culture were higher than those at 37°C in the differentiation culture, and S100, p75, and GFAP proteins were expressed more than at 37°C. GFAP and S100 were stained strongly at 35°C by immunohistochemical examination. These data demonstrate that culturing BM-MSCs at reduced temperature can improve differentiation into Schwann-like cells.

Original languageEnglish
Pages (from-to)259-267
Number of pages9
JournalTissue Engineering and Regenerative Medicine
Volume12
Issue number4
DOIs
StatePublished - 18 Aug 2015

Keywords

  • Bone marrow mesenchymal stem cell
  • Low temperature
  • Schwann-like cell differentiation

Fingerprint

Dive into the research topics of 'Effect of low temperature on Schwann-like cell differentiation of bone marrow mesenchymal stem cells'. Together they form a unique fingerprint.

Cite this