TY - JOUR
T1 - Effect of low temperature on Schwann-like cell differentiation of bone marrow mesenchymal stem cells
AU - Yoon, Hee Hoon
AU - Han, Mi Jung
AU - Park, Jung Keug
AU - Lee, Jong Ho
AU - Seo, Young Kwon
N1 - Publisher Copyright:
© 2015, The Korean Tissue Engineering and Regenerative Medicine Society and Springer Science+Business Media Dordrecht.
PY - 2015/8/18
Y1 - 2015/8/18
N2 - Mesenchymal stem cells (MSCs) are multipotent stromal cells that can differentiate into a variety of cell types. The potential of MSCs for differentiation and proliferation depends on the control of various processes. In this study, we have investigated the effects of low temperature on the Schwann-like cell differentiation of human bone marrow mesenchymal stem cells (BM-MSCs). The growth of BM-MSCs decreased at low temperature, but increased Schwann-like cell differentiation. The expression of cell surface antigen decreased CD73, CD105, and CD90, which are known markers of MSCs at 35°C. Also, the levels of matrix RNA [S100, glial fibrillary acidic protein (GFAP), p75, myelin basic protein, P0, and others] at 35°C in the differentiation culture were higher than those at 37°C in the differentiation culture, and S100, p75, and GFAP proteins were expressed more than at 37°C. GFAP and S100 were stained strongly at 35°C by immunohistochemical examination. These data demonstrate that culturing BM-MSCs at reduced temperature can improve differentiation into Schwann-like cells.
AB - Mesenchymal stem cells (MSCs) are multipotent stromal cells that can differentiate into a variety of cell types. The potential of MSCs for differentiation and proliferation depends on the control of various processes. In this study, we have investigated the effects of low temperature on the Schwann-like cell differentiation of human bone marrow mesenchymal stem cells (BM-MSCs). The growth of BM-MSCs decreased at low temperature, but increased Schwann-like cell differentiation. The expression of cell surface antigen decreased CD73, CD105, and CD90, which are known markers of MSCs at 35°C. Also, the levels of matrix RNA [S100, glial fibrillary acidic protein (GFAP), p75, myelin basic protein, P0, and others] at 35°C in the differentiation culture were higher than those at 37°C in the differentiation culture, and S100, p75, and GFAP proteins were expressed more than at 37°C. GFAP and S100 were stained strongly at 35°C by immunohistochemical examination. These data demonstrate that culturing BM-MSCs at reduced temperature can improve differentiation into Schwann-like cells.
KW - Bone marrow mesenchymal stem cell
KW - Low temperature
KW - Schwann-like cell differentiation
UR - http://www.scopus.com/inward/record.url?scp=84939130272&partnerID=8YFLogxK
U2 - 10.1007/s13770-014-0058-z
DO - 10.1007/s13770-014-0058-z
M3 - Article
AN - SCOPUS:84939130272
SN - 1738-2696
VL - 12
SP - 259
EP - 267
JO - Tissue Engineering and Regenerative Medicine
JF - Tissue Engineering and Regenerative Medicine
IS - 4
ER -