Effect of Magnetic Microparticles on Cultivated Human Corneal Endothelial Cells

Joo Hee Park, Kangmin Lee, Choul Yong Park

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Purpose: To investigate effects of magnetic microparticles on movement of magnet controlled human corneal endothelial cells (HCECs). Methods: Immortalized HCEC line (B4G12) and primary culture of HCECs were exposed to two commercially available magnetic micro-or nanoparticles, SiMAG (average size 100 nm) and fluidMAG (average size <1000 nm). Cell viability assays and reactive oxygen species production assays were performed. Cellular structural changes, intracel-lular distribution of microparticles, and expression levels of proteins related to cellular survival were analyzed. Ex vivo human corneas were exposed to microparticles to further evaluate their effects. Magnetic particle–laden HCECs were cultured under the influence of a neodymium magnet. Results: No significant decrease of viability was found in HCECs after exposure to both magnetic particles at concentrations up to 20 μg/mL for 48 hours. However, high concentrations (40 μg/mL and 80 μg/mL) of SiMAG and FluidMAG significantly decreased viability in immortalized HCECs, and only 80 μg/mL of SiMAG and FluidMAG decreased viability in primary HCECs after 48 hours of exposure. There was relative stabil-ity of viability at various concentrations of magnetic particles, despite a dose-dependent increase of reactive oxygen species, lactate dehydrogenase, and markers of apoptosis. Ex vivo human cornea study further revealed that exposure to 20 μg/mL of SiMAG and fluidMAG for 72 hours was tolerable. Endocytosed magnetic particles were mainly localized in the cytoplasm. The application of a magnetic field during cell culture successfully demonstrated that magnetic particle–loaded HCECs moved toward the magnet area and that the population density of HCECs was significantly increased. Conclusions: We verified short-term effects of SiMAG and fluidMAG on HCECs and their ability to control movement of HCECs by an external magnetic field. Translational Relevance: A technology of applying magnetic particles to a human corneal endothelial cell culture and controlling the movement of cells to a desired area using a magnetic field could be used to increase cell density during cell culture or improve the localization of corneal endothelial cells injected into the anterior chamber to the back of the cornea.

Original languageEnglish
Article number14
JournalTranslational Vision Science and Technology
Volume12
Issue number2
DOIs
StatePublished - Feb 2023

Keywords

  • control
  • corneal endothelial cells
  • corneal transplantation
  • magnetic particle
  • nanoparticle

Fingerprint

Dive into the research topics of 'Effect of Magnetic Microparticles on Cultivated Human Corneal Endothelial Cells'. Together they form a unique fingerprint.

Cite this