Effect of replicated polymeric substrate with lotus surface structure on adipose-derived stem cell behaviors

Kyoung Je Cha, Kwang Sook Park, Sun Woong Kang, Byung Hyun Cha, Bong Kee Lee, In Bo Han, Dong Ah Shin, Dong Sung Kim, Soo Hong Lee

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

We fabricated polystyrene substrates with lotus leaf surface structure (LLSS) and investigated cell behaviors, including attachment, morphology, proliferation, and differentiation of adipose-derived stem cells (ASCs) on them. Compared to the flat substrate, the LLSS substrate induced higher cell attachment rate, but did not significantly change the cell proliferation rate. In addition, ASCs on the LLSS substrate exhibited relatively narrower spreading morphology and less organized cytoskeleton, there by resulting in smaller sizes of cells than those on the flat substrate. According to histochemical staining and RT-PCR analysis, the LLSS substrate induced higher adipogenic differentiation of ASCs than the flat substrate, while chondrogenic and osteogenic differentiation were decreased.

Original languageEnglish
Pages (from-to)1357-1363
Number of pages7
JournalMacromolecular Bioscience
Volume11
Issue number10
DOIs
StatePublished - 10 Oct 2011

Keywords

  • Adipose-derived stem cell
  • Cell adhesion
  • Lotus leaf surface structure
  • Polymer replication
  • Stem cell differentiation

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