Effects of a hypomagnetic field on DNA methylation during the differentiation of embryonic stem cells

Soonbong Baek; Hwan Choi; Hanseul Park; Byunguk Cho; Siyoung Kim; Jongpil Kim

doi:10.1038/s41598-018-37372-2

Effects of a hypomagnetic field on DNA methylation during the differentiation of embryonic stem cells

Soonbong Baek, Hwan Choi, Hanseul Park, Byunguk Cho, Siyoung Kim, Jongpil Kim

Department of Chemistry

Dongguk University

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

It has been reported that hypomagnetic fields (HMFs) have a negative influence on mammalian physiological functions. We previously reported that HMFs were detrimental to cell fate changes during reprogramming into pluripotency. These studies led us to investigate whether HMFs affect cell fate determination during direct differentiation. Here, we found that an HMF environment attenuates differentiation capacity and is detrimental to cell fate changes during the in vitro differentiation of embryonic stem cells (ESCs). Moreover, HMF conditions cause abnormal DNA methylation through the dysregulation of DNA methyltransferase3b (Dnmt3b) expression, eventually resulting in incomplete DNA methylation during differentiation. Taken together, these results suggest that an appropriate electromagnetic field (EMF) environment may be essential for favorable epigenetic remodeling during cell fate determination via differentiation.

Original language	English
Article number	1333
Journal	Scientific Reports
Volume	9
Issue number	1
DOIs	https://doi.org/10.1038/s41598-018-37372-2
State	Published - 1 Dec 2019

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abstract = "It has been reported that hypomagnetic fields (HMFs) have a negative influence on mammalian physiological functions. We previously reported that HMFs were detrimental to cell fate changes during reprogramming into pluripotency. These studies led us to investigate whether HMFs affect cell fate determination during direct differentiation. Here, we found that an HMF environment attenuates differentiation capacity and is detrimental to cell fate changes during the in vitro differentiation of embryonic stem cells (ESCs). Moreover, HMF conditions cause abnormal DNA methylation through the dysregulation of DNA methyltransferase3b (Dnmt3b) expression, eventually resulting in incomplete DNA methylation during differentiation. Taken together, these results suggest that an appropriate electromagnetic field (EMF) environment may be essential for favorable epigenetic remodeling during cell fate determination via differentiation.",

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AU - Kim, Siyoung

AU - Kim, Jongpil

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Effects of a hypomagnetic field on DNA methylation during the differentiation of embryonic stem cells

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