Effects of human adipose-derived stem cells on cutaneous wound healing in nude mice

Seung Ho Lee, Joon Ho Lee, Kwang Hyun Cho

Research output: Contribution to journalArticlepeer-review

72 Scopus citations

Abstract

Background: Despite numerous treatments available for deteriorated cutaneous wound healing such as a diabetic foot, there is still the need for more effective therapy. Adipose-derived stem cells (ASCs) are mesenchymal stem cells, which are self-renewing and multipotent. Mesenchymal stem cells have the potential for tissue repair and regeneration. Objective: To investigate the effects of human ASCs on the healing of cutaneous wounds in nude mice. Methods: 15-mm round full-thickness skin defects were generated on the back of BALB/c nude mice. The mice were divided into three groups for wound coverage: (i) human ASCs-populated collagen gel, (ii) human dermal fibroblastspopulated collagen gel, and (iii) collagen gel alone. Wound contraction was prevented with a splint method. Wound size was measured 10 days after injury. At 28 days histological analysis was performed. Results: Both ASCs and dermal fibroblasts accelerated wound closure, but dermal fibroblasts were more effective than ASCs. At 28 days, the dermal portion of ASCs or dermal fibroblasts wound scars were thicker than collagen gel wound scars. Conclusion: ASCs and dermal fibroblasts stimulate cutaneous wound healing and improve scar thickness.

Original languageEnglish
Pages (from-to)150-155
Number of pages6
JournalAnnals of Dermatology
Volume23
Issue number2
DOIs
StatePublished - May 2011

Keywords

  • Adipose tissue
  • Fibroblast
  • Mesenchymal stem cell
  • Mouse
  • Wound healing

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