Abstract
Recent reports have suggested that vibration has beneficial effects on knee healing response; however, the biomechanism of these beneficial effects still need to be determined on the anterior cruciate ligament (ACL) cell level. In this study, we applied a 20 Hz vibration to ACL cells, which produced a 20% increase (P< 0.001) in cell activity and 17% increase (P< 0.001) in intracellular sulfated glycosaminoglycan levels. In the 20 Hz vibration-stimulated ACL cell group, eight up-regulated (100 ~ 300%) protein spots were identified compared with the control group by proteomics analysis. Among these proteins, Annexin A2 and Prolyl 4 hydroxylase (PH4B) were shown to have a 71% and 16% higher expression, respectively, in the 20 Hz vibration-stimulated ACL cell group by Western blotting (P< 0.001). These results indicate that vibration produces a positive cellular environment, and Annexin A2 and prolyl 4 hydroxylase are expected to help ligament repair and ACL cell proliferation by controlling cell membrane and extracellular matrix formation.
Original language | English |
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Pages (from-to) | 783-789 |
Number of pages | 7 |
Journal | Experimental Biology and Medicine |
Volume | 236 |
Issue number | 7 |
DOIs | |
State | Published - Jul 2011 |
Keywords
- Annexin A2
- Anterior cruciate ligament
- Extracellular matrix
- Prolyl 4 hydroxylase
- Vibration