Abstract
An efficient and wash-free method to conjugate a fluorescent tag to a target membrane protein is developed, using engineered Npu DnaE split-inteins. This approach allowed fast labeling while avoiding the strenuous synthesis of a long polypeptide. Two different modes of labeling, namely specific binding and covalent conjugation, are observed. The covalent labeling was monitored within 5 min, without background staining.
Original language | English |
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Pages (from-to) | 1568-1574 |
Number of pages | 7 |
Journal | Protein Science |
Volume | 27 |
Issue number | 9 |
DOIs | |
State | Published - Sep 2018 |
Keywords
- protein engineering
- protein labeling
- protein trans-splicing
- split-intein