Efficient and wash-free labeling of membrane proteins using engineered Npu DnaE split-inteins

Euiyeon Lee, Kyoungmi Min, Young Tae Chang, Youngeun Kwon

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

An efficient and wash-free method to conjugate a fluorescent tag to a target membrane protein is developed, using engineered Npu DnaE split-inteins. This approach allowed fast labeling while avoiding the strenuous synthesis of a long polypeptide. Two different modes of labeling, namely specific binding and covalent conjugation, are observed. The covalent labeling was monitored within 5 min, without background staining.

Original languageEnglish
Pages (from-to)1568-1574
Number of pages7
JournalProtein Science
Volume27
Issue number9
DOIs
StatePublished - Sep 2018

Keywords

  • protein engineering
  • protein labeling
  • protein trans-splicing
  • split-intein

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