Efficient biofilms eradication by enzymatic-cocktail of pancreatic protease type-i and bacterial α-amylase

Seung Cheol Jee, Min Kim, Jung Suk Sung, Avinash A. Kadam

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Removal of biofilms is extremely pivotal in environmental and medicinal fields. Therefore, reporting the new-enzymes and their combinations for dispersal of infectious biofilms can be extremely critical. Herein, for the first time, we accessed the enzyme “protease from bovine pancreas type-I (PtI)” for anti-biofilm properties. We further investigated the anti-biofilm potential of PtI in combination with α-amylase from Bacillus sp. (αA). PtI showed a very significant biofilm inhibition effect (86.5%, 88.4%, and 67%) and biofilm prevention effect (66%, 64%, and 70%), against the E. coli, S. aureus, and MRSA, respectively. However, the new enzyme combination (Ec-PtI+αA) exhibited biofilm inhibition effect (78%, 90%, and 93%) and a biofilm prevention effect (44%, 51%, and 77%) against E. coli, S. aureus, and MRSA, respectively. The studied enzymes were found not to be anti-bacterial against the E. coli, S. aureus, and MRSA. In summary, the PtI exhibited significant anti-biofilm effects against S. aureus, MRSA, and E. coli. Ec-PtI+αA exhibited enhancement of the anti-biofilm effects against S. aureus and MRSA biofilms. Therefore, this study revealed that this Ec-PtI+αA enzymatic system can be extremely vital for the treatment of biofilm complications resulting from E. coli, S. aureus, and MRSA.

Original languageEnglish
Article number3032
Pages (from-to)1-13
Number of pages13
JournalPolymers
Volume12
Issue number12
DOIs
StatePublished - Dec 2020

Keywords

  • Anti-biofilm enzymes
  • Biofilm eradication
  • Protease type-I
  • α-amylase

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