TY - JOUR
T1 - Efficient degradation and detoxification of methylene blue dye by a newly isolated ligninolytic enzyme producing bacterium Bacillus albus MW407057
AU - Kishor, Roop
AU - Saratale, Ganesh Dattatraya
AU - Saratale, Rijuta Ganesh
AU - Romanholo Ferreira, Luiz Fernando
AU - Bilal, Muhammad
AU - Iqbal, Hafiz M.N.
AU - Bharagava, Ram Naresh
N1 - Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2021/10
Y1 - 2021/10
N2 - In present work, a LiP enzyme producing bacterium was isolated form textile wastewater and sludge sample and identified as Bacillus albus by 16S rRNA gene sequencing analysis. This bacterium decolorized 99.27 % MB dye and removed 83.87 % COD within 6 h at 30 °C, pH 7, 100 rpm and 100 mg/l of dye concentration in presence of glucose and yeast extract as carbon and nitrogen source, respectively. The bacterium also produced LiP enzyme of molecular weight ∼48 kDa, characterized by SDS-PAGE analysis. Different metabolites like monomethylthionine, thionin, (E)-2-(3-Oxopropylidene)-2H-benzo[b][1,4] thiozine-3-carboxylic acid, N-(3,4-dihydroxyphenyl)-N-methylformamide, ethylamine, water and carbon dioxide produced during treatment process were characterized by FT-IR and LC–MS analysis. Further, the toxicity assessment results showed that the toxicity of bacteria treated dye solution was reduced significantly allowing 90 % seed germination indicating that the isolated bacterium B. albus has high potential to decolorize and detoxify MB dye for environmental safety.
AB - In present work, a LiP enzyme producing bacterium was isolated form textile wastewater and sludge sample and identified as Bacillus albus by 16S rRNA gene sequencing analysis. This bacterium decolorized 99.27 % MB dye and removed 83.87 % COD within 6 h at 30 °C, pH 7, 100 rpm and 100 mg/l of dye concentration in presence of glucose and yeast extract as carbon and nitrogen source, respectively. The bacterium also produced LiP enzyme of molecular weight ∼48 kDa, characterized by SDS-PAGE analysis. Different metabolites like monomethylthionine, thionin, (E)-2-(3-Oxopropylidene)-2H-benzo[b][1,4] thiozine-3-carboxylic acid, N-(3,4-dihydroxyphenyl)-N-methylformamide, ethylamine, water and carbon dioxide produced during treatment process were characterized by FT-IR and LC–MS analysis. Further, the toxicity assessment results showed that the toxicity of bacteria treated dye solution was reduced significantly allowing 90 % seed germination indicating that the isolated bacterium B. albus has high potential to decolorize and detoxify MB dye for environmental safety.
KW - Degradation
KW - LC–MS analysis
KW - Lignin peroxidase
KW - Methylene blue
KW - Phytotoxicity
UR - http://www.scopus.com/inward/record.url?scp=85109460720&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfb.2021.111947
DO - 10.1016/j.colsurfb.2021.111947
M3 - Article
C2 - 34218015
AN - SCOPUS:85109460720
SN - 0927-7765
VL - 206
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
M1 - 111947
ER -