Efficient degradation and detoxification of methylene blue dye by a newly isolated ligninolytic enzyme producing bacterium Bacillus albus MW407057

Roop Kishor, Ganesh Dattatraya Saratale, Rijuta Ganesh Saratale, Luiz Fernando Romanholo Ferreira, Muhammad Bilal, Hafiz M.N. Iqbal, Ram Naresh Bharagava

Research output: Contribution to journalArticlepeer-review

77 Scopus citations

Abstract

In present work, a LiP enzyme producing bacterium was isolated form textile wastewater and sludge sample and identified as Bacillus albus by 16S rRNA gene sequencing analysis. This bacterium decolorized 99.27 % MB dye and removed 83.87 % COD within 6 h at 30 °C, pH 7, 100 rpm and 100 mg/l of dye concentration in presence of glucose and yeast extract as carbon and nitrogen source, respectively. The bacterium also produced LiP enzyme of molecular weight ∼48 kDa, characterized by SDS-PAGE analysis. Different metabolites like monomethylthionine, thionin, (E)-2-(3-Oxopropylidene)-2H-benzo[b][1,4] thiozine-3-carboxylic acid, N-(3,4-dihydroxyphenyl)-N-methylformamide, ethylamine, water and carbon dioxide produced during treatment process were characterized by FT-IR and LC–MS analysis. Further, the toxicity assessment results showed that the toxicity of bacteria treated dye solution was reduced significantly allowing 90 % seed germination indicating that the isolated bacterium B. albus has high potential to decolorize and detoxify MB dye for environmental safety.

Original languageEnglish
Article number111947
JournalColloids and Surfaces B: Biointerfaces
Volume206
DOIs
StatePublished - Oct 2021

Keywords

  • Degradation
  • LC–MS analysis
  • Lignin peroxidase
  • Methylene blue
  • Phytotoxicity

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