Efficient direct lineage reprogramming of fibroblasts into induced cardiomyocytes using nanotopographical cues

Junsang Yoo; Yujung Chang; Hongwon Kim; Soonbong Baek; Hwan Choi; Gun Jae Jeong; Jaein Shin; Hongnam Kim; Byung Soo Kim; Jongpil Kim

doi:10.1166/jbn.2017.2347

Efficient direct lineage reprogramming of fibroblasts into induced cardiomyocytes using nanotopographical cues

Junsang Yoo, Yujung Chang, Hongwon Kim, Soonbong Baek, Hwan Choi, Gun Jae Jeong, Jaein Shin, Hongnam Kim, Byung Soo Kim, Jongpil Kim

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Induced cardiomyocytes (iCMs) generated via direct lineage reprogramming offer a novel therapeutic target for the study and treatment of cardiac diseases. However, the efficiency of iCM generation is significantly low for therapeutic applications. Here, we show an efficient direct conversion of somatic fibroblasts into iCMs using nanotopographic cues. Compared with flat substrates, the direct conversion of fibroblasts into iCMs on nanopatterned substrates resulted in a dramatic increase in the reprogramming efficiency and maturation of iCM phenotypes. Additionally, enhanced reprogramming by substrate nanotopography was due to changes in the activation of focal adhesion kinase and specific histone modifications. Taken together, these results suggest that nanotopographic cues can serve as an efficient stimulant for direct lineage reprogramming into iCMs.

Original language	English
Pages (from-to)	269-279
Number of pages	11
Journal	Journal of Biomedical Nanotechnology
Volume	13
Issue number	3
DOIs	https://doi.org/10.1166/jbn.2017.2347
State	Published - Mar 2017

Keywords

Direct Lineage Reprogramming
Induced Cardiomyocytes
Nanotopographical Cues

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10.1166/jbn.2017.2347

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title = "Efficient direct lineage reprogramming of fibroblasts into induced cardiomyocytes using nanotopographical cues",

abstract = "Induced cardiomyocytes (iCMs) generated via direct lineage reprogramming offer a novel therapeutic target for the study and treatment of cardiac diseases. However, the efficiency of iCM generation is significantly low for therapeutic applications. Here, we show an efficient direct conversion of somatic fibroblasts into iCMs using nanotopographic cues. Compared with flat substrates, the direct conversion of fibroblasts into iCMs on nanopatterned substrates resulted in a dramatic increase in the reprogramming efficiency and maturation of iCM phenotypes. Additionally, enhanced reprogramming by substrate nanotopography was due to changes in the activation of focal adhesion kinase and specific histone modifications. Taken together, these results suggest that nanotopographic cues can serve as an efficient stimulant for direct lineage reprogramming into iCMs.",

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author = "Junsang Yoo and Yujung Chang and Hongwon Kim and Soonbong Baek and Hwan Choi and Jeong, {Gun Jae} and Jaein Shin and Hongnam Kim and Kim, {Byung Soo} and Jongpil Kim",

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AU - Yoo, Junsang

AU - Chang, Yujung

AU - Kim, Hongwon

AU - Baek, Soonbong

AU - Choi, Hwan

AU - Jeong, Gun Jae

AU - Shin, Jaein

AU - Kim, Hongnam

AU - Kim, Byung Soo

AU - Kim, Jongpil

PY - 2017/3

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N2 - Induced cardiomyocytes (iCMs) generated via direct lineage reprogramming offer a novel therapeutic target for the study and treatment of cardiac diseases. However, the efficiency of iCM generation is significantly low for therapeutic applications. Here, we show an efficient direct conversion of somatic fibroblasts into iCMs using nanotopographic cues. Compared with flat substrates, the direct conversion of fibroblasts into iCMs on nanopatterned substrates resulted in a dramatic increase in the reprogramming efficiency and maturation of iCM phenotypes. Additionally, enhanced reprogramming by substrate nanotopography was due to changes in the activation of focal adhesion kinase and specific histone modifications. Taken together, these results suggest that nanotopographic cues can serve as an efficient stimulant for direct lineage reprogramming into iCMs.

AB - Induced cardiomyocytes (iCMs) generated via direct lineage reprogramming offer a novel therapeutic target for the study and treatment of cardiac diseases. However, the efficiency of iCM generation is significantly low for therapeutic applications. Here, we show an efficient direct conversion of somatic fibroblasts into iCMs using nanotopographic cues. Compared with flat substrates, the direct conversion of fibroblasts into iCMs on nanopatterned substrates resulted in a dramatic increase in the reprogramming efficiency and maturation of iCM phenotypes. Additionally, enhanced reprogramming by substrate nanotopography was due to changes in the activation of focal adhesion kinase and specific histone modifications. Taken together, these results suggest that nanotopographic cues can serve as an efficient stimulant for direct lineage reprogramming into iCMs.

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KW - Nanotopographical Cues

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Efficient direct lineage reprogramming of fibroblasts into induced cardiomyocytes using nanotopographical cues

Abstract

Keywords

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