Electrically tethered DNA stretching in nanochannels

Byung Chul Lee, Chanmin Kang, Jinsik Kim, Ji Yoon Kang, Hyun Joon Shin, Sang Youp Lee

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

In present study, stretching dynamics of electrically tethered λ-DNA (48.5kbp) in SiO2 nanochannels has been investigated. At high electrical fields (above 20kV/m), elongations of electrically tethered DNA molecules were observed. At high E-fields, DNAs were tethered in nanochannels and were spontaneously elongated along the nanochannels up to about 90 percent of its contour length. With E-field turned off, the measured relaxation time was about 10 sec from stretching with 20kV/m. In current study, observed behaviors of DNA molecules in nanochannels were explained by field-induced dielectrophoretic DNA trap due to the particular cross-sectional geometry of nanochannels. Also the elongation ratio between 20kV/m and 60kV/m cases and the effect of E-field distribution in the transverse plane on fieldinduced dielectrophoretic tethering force are discussed based on "worm-like chain" model. The FEM simulation was done to verify induced dielectrophoretic tethering force into the nanohorn.

Original languageEnglish
Title of host publicationProceedings of the ASME International Mechanical Engineering Congress and Exposition 2009, IMECE 2009
PublisherAmerican Society of Mechanical Engineers (ASME)
Pages655-661
Number of pages7
EditionPART B
ISBN (Print)9780791843857
DOIs
StatePublished - 2010
EventASME 2009 International Mechanical Engineering Congress and Exposition, IMECE2009 - Lake Buena Vista, FL, United States
Duration: 13 Nov 200919 Nov 2009

Publication series

NameASME International Mechanical Engineering Congress and Exposition, Proceedings
NumberPART B
Volume12

Conference

ConferenceASME 2009 International Mechanical Engineering Congress and Exposition, IMECE2009
Country/TerritoryUnited States
CityLake Buena Vista, FL
Period13/11/0919/11/09

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