TY - JOUR
T1 - Engineered whole-cell biocatalyst-based detoxification and detection of neurotoxic organophosphate compounds
AU - Kim, Chang Sup
AU - Seo, Jeong Hyun
AU - Kang, Dong Gyun
AU - Cha, Hyung Joon
PY - 2014/5
Y1 - 2014/5
N2 - The development of efficient tools is required for the eco-friendly detoxification and effective detection of neurotoxic organophosphates (OPs). Although enzymes have received significant attention as biocatalysts because of their high specific activity, the uneconomic and labor-intensive processes of enzyme production and purification make their broad use in practical applications difficult. Because whole-cell systems offer several advantages compared with free enzymes, including high stability, a reduced purification requirement, and low preparation cost, they have been suggested as promising biocatalysts for the detoxification and detection of OPs. To develop efficient whole-cell biocatalysts with enhanced activity and a broad spectrum of substrate specificity, several factors have been considered, namely the selected strains, the chosen OP-hydrolyzing enzymes, where enzymes are localized in a cell, and which enhancer will assist the expression, function, and folding of the enzyme. In this article, we review the current investigative progress in the development of engineered whole-cell biocatalysts with excellent OP-hydrolyzing activity, a broad spectrum of substrate specificity, and outstanding stability for the detoxification and detection of OPs.
AB - The development of efficient tools is required for the eco-friendly detoxification and effective detection of neurotoxic organophosphates (OPs). Although enzymes have received significant attention as biocatalysts because of their high specific activity, the uneconomic and labor-intensive processes of enzyme production and purification make their broad use in practical applications difficult. Because whole-cell systems offer several advantages compared with free enzymes, including high stability, a reduced purification requirement, and low preparation cost, they have been suggested as promising biocatalysts for the detoxification and detection of OPs. To develop efficient whole-cell biocatalysts with enhanced activity and a broad spectrum of substrate specificity, several factors have been considered, namely the selected strains, the chosen OP-hydrolyzing enzymes, where enzymes are localized in a cell, and which enhancer will assist the expression, function, and folding of the enzyme. In this article, we review the current investigative progress in the development of engineered whole-cell biocatalysts with excellent OP-hydrolyzing activity, a broad spectrum of substrate specificity, and outstanding stability for the detoxification and detection of OPs.
KW - Detection
KW - Detoxification
KW - Organophosphate-hydrolyzing enzyme
KW - Organophosphates
KW - Whole-cell biocatalyst
UR - http://www.scopus.com/inward/record.url?scp=84900563223&partnerID=8YFLogxK
U2 - 10.1016/j.biotechadv.2014.04.010
DO - 10.1016/j.biotechadv.2014.04.010
M3 - Review article
C2 - 24780157
AN - SCOPUS:84900563223
SN - 0734-9750
VL - 32
SP - 652
EP - 662
JO - Biotechnology Advances
JF - Biotechnology Advances
IS - 3
ER -