Engineered whole-cell biocatalyst-based detoxification and detection of neurotoxic organophosphate compounds

Chang Sup Kim, Jeong Hyun Seo, Dong Gyun Kang, Hyung Joon Cha

Research output: Contribution to journalReview articlepeer-review

19 Scopus citations

Abstract

The development of efficient tools is required for the eco-friendly detoxification and effective detection of neurotoxic organophosphates (OPs). Although enzymes have received significant attention as biocatalysts because of their high specific activity, the uneconomic and labor-intensive processes of enzyme production and purification make their broad use in practical applications difficult. Because whole-cell systems offer several advantages compared with free enzymes, including high stability, a reduced purification requirement, and low preparation cost, they have been suggested as promising biocatalysts for the detoxification and detection of OPs. To develop efficient whole-cell biocatalysts with enhanced activity and a broad spectrum of substrate specificity, several factors have been considered, namely the selected strains, the chosen OP-hydrolyzing enzymes, where enzymes are localized in a cell, and which enhancer will assist the expression, function, and folding of the enzyme. In this article, we review the current investigative progress in the development of engineered whole-cell biocatalysts with excellent OP-hydrolyzing activity, a broad spectrum of substrate specificity, and outstanding stability for the detoxification and detection of OPs.

Original languageEnglish
Pages (from-to)652-662
Number of pages11
JournalBiotechnology Advances
Volume32
Issue number3
DOIs
StatePublished - May 2014

Keywords

  • Detection
  • Detoxification
  • Organophosphate-hydrolyzing enzyme
  • Organophosphates
  • Whole-cell biocatalyst

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