Engineering three-dimensional neural circuit with neurite bundle like structure by hydrogel alignment

Sang Cheol Na, Seokyoung Bang, Jae Myung Jang, Noo Li Jeon

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

We report a microfluidic model of a 3D neural circuit. The microfluidic device was filled with Matrigel and continuous flow was provided across the device during gelation. The fluidic flow aligned the ECM components along the flow direction. Following the alignment of ECM fibers, neurites of primary rat cortical neurons were grown into the deformed Matrigel at the average speed of 254.2 μm/day, and formed axon bundles approximately 1500 μm in length at 6 DIV. Additionally, neural networks were developed from pre-synaptic to post-synaptic channels at 14 DIV. The establishment of aligned 3D neural circuits was confirmed with the immunostaining and the quantification of calcium signal transmission.

Original languageEnglish
Title of host publicationMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages576-578
Number of pages3
ISBN (Electronic)9780979806483
StatePublished - 2015
Event19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of
Duration: 25 Oct 201529 Oct 2015

Publication series

NameMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
Country/TerritoryKorea, Republic of
CityGyeongju
Period25/10/1529/10/15

Keywords

  • 3D neuron culture
  • Microfluidic
  • Neural circuit

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