@inproceedings{7a81d49744434d1184484088e20396d1,
title = "Engineering three-dimensional neural circuit with neurite bundle like structure by hydrogel alignment",
abstract = "We report a microfluidic model of a 3D neural circuit. The microfluidic device was filled with Matrigel and continuous flow was provided across the device during gelation. The fluidic flow aligned the ECM components along the flow direction. Following the alignment of ECM fibers, neurites of primary rat cortical neurons were grown into the deformed Matrigel at the average speed of 254.2 μm/day, and formed axon bundles approximately 1500 μm in length at 6 DIV. Additionally, neural networks were developed from pre-synaptic to post-synaptic channels at 14 DIV. The establishment of aligned 3D neural circuits was confirmed with the immunostaining and the quantification of calcium signal transmission.",
keywords = "3D neuron culture, Microfluidic, Neural circuit",
author = "Na, {Sang Cheol} and Seokyoung Bang and Jang, {Jae Myung} and Jeon, {Noo Li}",
note = "Publisher Copyright: {\textcopyright} 15CBMS-0001.; 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 ; Conference date: 25-10-2015 Through 29-10-2015",
year = "2015",
language = "English",
series = "MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
publisher = "Chemical and Biological Microsystems Society",
pages = "576--578",
booktitle = "MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
}