Enhanced immunodetection of cell-free synthesized erythropoietin under denaturing conditions

Jin Kang Taek, Kyoung Song Hui, Ho Ahn Jin, Wan Kim Tae, Yeo Joon Yeo, Yong Choi Cha

Research output: Contribution to journalArticlepeer-review

Abstract

A monoclonal antibody produced by hydridoma cell line, ATCC HB8209, was used to detect and purify erythropoietin synthesized in a cell-free system. The antibody was raised against the N-terminal 20 residues of erythropoietin. It retained anti-erythropoietin activity in 6 M urea in which most of the cell-free synthesized erythropoietin became soluble and gave an enhanced activity of the antibody.

Original languageEnglish
Pages (from-to)641-644
Number of pages4
JournalBiotechnology Letters
Volume26
Issue number8
DOIs
StatePublished - Apr 2004

Keywords

  • Denaturing condition
  • Epitope tag
  • Erythropoietin
  • Immunoaffinity

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