Abstract
A monoclonal antibody produced by hydridoma cell line, ATCC HB8209, was used to detect and purify erythropoietin synthesized in a cell-free system. The antibody was raised against the N-terminal 20 residues of erythropoietin. It retained anti-erythropoietin activity in 6 M urea in which most of the cell-free synthesized erythropoietin became soluble and gave an enhanced activity of the antibody.
Original language | English |
---|---|
Pages (from-to) | 641-644 |
Number of pages | 4 |
Journal | Biotechnology Letters |
Volume | 26 |
Issue number | 8 |
DOIs | |
State | Published - Apr 2004 |
Keywords
- Denaturing condition
- Epitope tag
- Erythropoietin
- Immunoaffinity