Enhanced immunodetection of cell-free synthesized erythropoietin under denaturing conditions

Jin Kang Taek; Kyoung Song Hui; Ho Ahn Jin; Wan Kim Tae; Yeo Joon Yeo; Yong Choi Cha

doi:10.1023/B:BILE.0000023022.60035.1e

Enhanced immunodetection of cell-free synthesized erythropoietin under denaturing conditions

Jin Kang Taek
, Kyoung Song Hui
, Ho Ahn Jin
, Wan Kim Tae
, Yeo Joon Yeo
, Yong Choi Cha

Department of Chemical and Biochemical Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

A monoclonal antibody produced by hydridoma cell line, ATCC HB8209, was used to detect and purify erythropoietin synthesized in a cell-free system. The antibody was raised against the N-terminal 20 residues of erythropoietin. It retained anti-erythropoietin activity in 6 M urea in which most of the cell-free synthesized erythropoietin became soluble and gave an enhanced activity of the antibody.

Original language	English
Pages (from-to)	641-644
Number of pages	4
Journal	Biotechnology Letters
Volume	26
Issue number	8
DOIs	https://doi.org/10.1023/B:BILE.0000023022.60035.1e
State	Published - Apr 2004

Keywords

Denaturing condition
Epitope tag
Erythropoietin
Immunoaffinity

Access to Document

10.1023/B:BILE.0000023022.60035.1e

Cite this

@article{beafc71b21ba4ca496b242d6ca4ad2b7,

title = "Enhanced immunodetection of cell-free synthesized erythropoietin under denaturing conditions",

abstract = "A monoclonal antibody produced by hydridoma cell line, ATCC HB8209, was used to detect and purify erythropoietin synthesized in a cell-free system. The antibody was raised against the N-terminal 20 residues of erythropoietin. It retained anti-erythropoietin activity in 6 M urea in which most of the cell-free synthesized erythropoietin became soluble and gave an enhanced activity of the antibody.",

keywords = "Denaturing condition, Epitope tag, Erythropoietin, Immunoaffinity",

author = "Taek, \{Jin Kang\} and Hui, \{Kyoung Song\} and Jin, \{Ho Ahn\} and Tae, \{Wan Kim\} and Yeo, \{Yeo Joon\} and Cha, \{Yong Choi\}",

year = "2004",

month = apr,

doi = "10.1023/B:BILE.0000023022.60035.1e",

language = "English",

volume = "26",

pages = "641--644",

journal = "Biotechnology Letters",

issn = "0141-5492",

publisher = "Springer Science and Business Media B.V.",

number = "8",

}

TY - JOUR

T1 - Enhanced immunodetection of cell-free synthesized erythropoietin under denaturing conditions

AU - Taek, Jin Kang

AU - Hui, Kyoung Song

AU - Jin, Ho Ahn

AU - Tae, Wan Kim

AU - Yeo, Yeo Joon

AU - Cha, Yong Choi

PY - 2004/4

Y1 - 2004/4

N2 - A monoclonal antibody produced by hydridoma cell line, ATCC HB8209, was used to detect and purify erythropoietin synthesized in a cell-free system. The antibody was raised against the N-terminal 20 residues of erythropoietin. It retained anti-erythropoietin activity in 6 M urea in which most of the cell-free synthesized erythropoietin became soluble and gave an enhanced activity of the antibody.

AB - A monoclonal antibody produced by hydridoma cell line, ATCC HB8209, was used to detect and purify erythropoietin synthesized in a cell-free system. The antibody was raised against the N-terminal 20 residues of erythropoietin. It retained anti-erythropoietin activity in 6 M urea in which most of the cell-free synthesized erythropoietin became soluble and gave an enhanced activity of the antibody.

KW - Denaturing condition

KW - Epitope tag

KW - Erythropoietin

KW - Immunoaffinity

UR - https://www.scopus.com/pages/publications/3342923528

U2 - 10.1023/B:BILE.0000023022.60035.1e

DO - 10.1023/B:BILE.0000023022.60035.1e

M3 - Article

C2 - 15200173

AN - SCOPUS:3342923528

SN - 0141-5492

VL - 26

SP - 641

EP - 644

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 8

ER -

Enhanced immunodetection of cell-free synthesized erythropoietin under denaturing conditions

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this