Abstract
Platelet-rich plasma (PRP) contains growth factors that promote tissue regeneration. Previously, we showed that heparin-conjugated fibrin (HCF) exerts the sustained release of growth factors with affinity for heparin. Here, we hypothesize that treatment of skin wound with a mixture of PRP and HCF exerts sustained release of several growth factors contained in PRP and promotes skin wound healing. The release of fibroblast growth factor 2, platelet-derived growth factor-BB, and vascular endothelial growth factor contained in PRP from HCF was sustained for a longer period than those from PRP, calcium-activated PRP (C-PRP), or a mixture of fibrin and PRP (F-PRP). Treatment of full-thickness skin wounds in mice with HCF-PRP resulted in much faster wound closure as well as dermal and epidermal regeneration at day 12 compared to treatment with either C-PRP or F-PRP. Enhanced skin regeneration observed in HCF-PRP group may have been at least partially due to enhanced angiogenesis in the wound beds. Therefore, this method could be useful for skin wound treatment.
Original language | English |
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Pages (from-to) | 622-629 |
Number of pages | 8 |
Journal | Experimental and Molecular Medicine |
Volume | 43 |
Issue number | 11 |
DOIs | |
State | Published - 2011 |
Keywords
- Angiogenesis inducing agents
- Endothelial growth factors
- Fibrin
- Fibroblast growth factor 2
- Heparin
- Neovascularization
- Physiologic
- Platelet-rich plasma
- Plateletderived growth factor
- Wound healing